TY - JOUR AU - Hernández-Morales, Javier AU - Romero-Rosales, Teolincacihuatl AU - Michel-Aceves, Alejandro AU - Vargas-Hernández, Mateo AU - Monteon-Ojeda, Abraham AU - Valenzuela-Lagarda, José PY - 2021/08/24 Y2 - 2024/03/28 TI - Enzymatic antagonism of Trichoderma spp., on hytophthora parasitica and Fusarium oxysporum in jamaica (Hibiscus sabdariffa L.) JF - Bioagro JA - Bioagro VL - 33 IS - 3 SE - DO - 10.51372/bioagro333.6 UR - https://revistas.uclave.org/index.php/bioagro/article/view/3384 SP - 203-214 AB - <p>Mexico is the seventh largest producer in Jamaica and Guerrero agglomerates more than 70% of the national production. The crop presents important phytosanitary limitations, highlights the "black leg" associated with a complex of pathogens (<em>Phytophthora parasitica</em> and <em>Fusarium oxysporum</em>). Losses up to 100% are reported in areas without phytosanitary management. Due to the nature of the consumption of the final product, it is necessary to implement ecological strategies to manage the disease. The objective of the study was to quantify the chitinases and glucanases activity of <em>Trichoderma</em> spp. Isolates, and to evaluate their inhibitory effect in vitro on <em>Phytophthora parasitica</em> and <em>Fusarium oxysporum</em>. Strains Ta10, Ta11, Ta6 and Ta9 of <em>T. asperellum</em> and Ti14 of <em>T. Inhamatum</em> from soils cultivated with hibiscus and high incidence of <em>P. parasitica</em> and <em>F. oxysporum</em> in Guerrero were evaluated. The levels of chitinases and glucanases produced in all <em>Trichoderma</em> spp. were significant (p£0,05), it could be observed that Ta9 from <em>T. asperellum</em> showed the highest specific chitinase activity, in the case of Ti14 glucanases from <em>T. inhamatum</em> it obtained the highest levels. All the filtrates from the different isolates of <em>Trichoderma</em> spp. generated a substantial inhibition of mycelial growth of <em>Fusarium oxysporum</em> and <em>Phytophthora parasitica</em>. Consistently Ta9 of <em>Trichoderma asperellum</em> obtained inhibition percentages greater than 90% in both tests. A significant correlation was detected between the enzymatic activity and the inhibition of the growth of the <em>Trichoderma</em> spp isolates. on pathogens.</p> ER -