Obtaining infecting larvated eggs of toxocara canis for inoculation of balb/c mice in experimental studies
Abstract
Infection with Toxocara canis (T. canis) represents an important parasitic zoonosis with worldwide distribution. Man becomes infected by involuntarily ingesting larvated eggs of the parasite. It is important to know the behavior of T. canis in different human organs, by carrying out experimental studies in mice of the BALB/c strain, for which it is necessary to infect them with viable larvated eggs of the parasite. The objective of this research was to obtain infective larvae eggs of Toxocara canis for inoculation of BALB/c mice in experimental studies. We worked with 12 adult females of T. canis, from a mixed-breed female dog (Lupus canis familiaris) puppy, with clinical signs of parasitosis and a positive coprological result for Toxocara spp., which was treated with pyrantel pamoate at a single dose of 15 mg/kg. orally. The uterus was dissected from these females and a total of 1.200.000 eggs of the parasite were obtained, which were incubated in 2.5% formalin/ringer's solution at a temperature of 25 °C and a humidity of 98% for 4 weeks, they were oxygenated and monitored every 3 days, observing the different phases of their development. From the first to the last review, a viability of 99 to 99.5% was found, that is, only 0.5 to 1% of the total eggs did not develop the larval form. The infective larvae eggs of T. canis represent the most important risk factor in the transmission of this disease.
Downloads
References
Delgado O, Rodríguez A. Aspectos clínico-epidemiológicos de la toxocariasis: una enfermedad desatendida en Venezuela y América Latina. Boletín de Malariología y Salud Ambiental 2009; 49(1):1-33.
McGuinness S, Leder, K. Global Burden of Toxocariasis: A Common Neglected Infection of Poverty. Current Tropical Medicine Reports 2014; 1(1):52-61.
Macpherson, C. The epidemiology and public health importance of toxocariosis: A zoonosis of global importance. International Journal for Parasitology 2013; 43(2013):999-1008.
Pinelli E, Aranzamendi C. Toxocara infection and its Association with Allergic Manifestations. Endocrine, Metabolic & Immune Disorders – Drug Targets 2012; 12:33-44.
Strube C, Heuer L, Janecek E. Toxocara spp infections in paratenic hosts Veterinary Parasitology 2013; 193(2013):375-389.
Quiroz, H. Parasitología y enfermedades parasitarias de animales domésticos. Grupo Noriega Editores México, DF. 2000.
Romero C, Yañez S, Mendoza G, Bustamante L, Ramírez N. Contaminación y viabilidad de huevos de Toxocara spp. en suelo y heces colectadas en parques públicos, calles y perros en Toluca, México. Revista Científica, FCV-LUZ 2013; 23(6):475-479.
García G, De Lima P, Mendonca M, Nunes A, McBride A, Scaini C. Human toxocariosis: current advances in diagnostics, treatment, and interventions. Trends in Parasitology 2014a; 30(9):456-464.
Acha P, Szyfres B. Zoonosis: Enfermedades transmisibles comunes al hombre y a los animales. 3 ed. Organización Panamericana de la Salud Washington, DC. 2003.
Camparoto M.L, Fulan B, Colli C.M, Paludo M.L, Falavigna-Guilherme A.L, Fernandez, M.A. Initial stage of development and migratory behavior of Toxocara canis larvae in BALB/c mouse experimental model. Genetics and Molecular Research 2008; 7(2):444-450.
Arroyo V, Siccha K, Terán V, Zafra L, Zeña L, Jara C. Efecto del extracto hidroalcohólico de Coriandrum sativum (Apiaceae) sobre la infectividad de huevos embrionados de Toxocara canis en Mus musculus BALB/c. REBIOLEST. Revista Científica de Estudiantes 2013; 1(1):37-42.
Rodrigues G, Vieira S, Chieffi P, Martins F, Borges R, Zevallos S. Experimental Toxocariosis in BALB/c mice: relationship between parasite inoculum and the IgG immune response. Memorias del Instituto Oswaldo Cruz 2017; 112(5):382-386.
Schoenardie E, Scaini C, Pepe M, Borsuk S, Da Costa L, Villela, M. Vertical transmission of Toxocara canis in successive generations of mice. Revista Brasileira de Parasitologia Veterinária, Jaboticabal 2013; 22(4):623-626.
Dutra G.F, Franca N.S, Da Costa L.F, Dutra P.C, Telmo P.L, Rodrigues L.H. Risk of infection by the consumption of liver of chickens inoculated with low doses of Toxocara canis eggs. Veterinary Parasitology 2014; 203:87–90
Telmo P.L, Avila L.F.C, Santos C.A, Aguiar P.S, Martins L.H.R, Berne M.E.A. Elevated trans-mammary transmission of Toxocara canis larvae in BALB/c mice. Revista del Instituto Medico Tropical de Sao Paulo 2015; 57(1):85-87.
Brunton L, Lazo J, Parker, K. Goodman y Gilman. Las bases farmacológicas de la terapéutica. 11 ed. McGraw-Hill Interamericana Mexico, D.F. 2007.
Lescano S, Santos S, Queiroz M, Castro J, Chieffi P. Migration routes of Toxocara canis and Toxocara cati in tissues of experimentally infected Rattus norvegicus. Neotropical Helminthology 2013; 7(1):149-153.
Zibaei M, Sadjjadi SM, Jahadi SH, Sarkari B. A Method for Accelerating the Maturation of Toxocara cati Eggs. Iranian Journal Parasitology 2007; 2(1):39-42
Sariego I, Pino A, Scull R, Cuéllar A, Fernández A, Rojas L. Actividad toxocaricida de plantas cubanas. Revista Cubana de Medicina Tropical 2015; 67(3):1-10.
Nassef N, El-Kersh W, El-Nahas N, Shams El-Din S, Oshiba S, Nosseir M. Parasitological, histopathological, and immunohistochemical assessment of nitric oxide synthase inhibitor: aminoguanidine versus albendazole in the treatment of experimental murine toxocariosis. Menoufia Medical Journal 2014; 27:103–114.
Santarém V, Silva C, Magosso L, Cavalcante L, Rubinsky G, Laposy C. Detection of larvae of Toxocara canis in milk: an experimental study in rabbits. Ciencias Agrarias, Londrina 2014; 35(1):357-364.
Chen J, Liu Q, Liu G, Zheng W, Hong S, Sugiyama H. Toxocariasis: a silent threat with a progressive public health impact. Infectious Diseases of Poverty 2018; 7(59):1-13.
Maleki B, Khorshidi A, Gorgipour M, Mirzapour A, Majidiani H, Foroutan M. Prevalence of Toxocara spp. Eggs in soil of public areas in Iran: A systematic review and meta-analysis. Alexandria Journal of Medicine 2018; 54:97-101
Abou-El-Naga, I. Developmental stages and viability of Toxocara canis eggs outside the host. Biomédica 2018; 38(2):1-26.
Baalbaki M, El Najjar M, Atweh S, El Ayoubi N. Toxocara infection in the differential diagnosis of multiple sclerosis in the Middle East. Multiple Sclerosis Journal-Experimental, Translational and Clinical. Enero-Marzo 2020; (2020):1-10.
Published
How to Cite
Issue
Section
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Gaceta de Ciencias Veterinarias se apega al modelo Open Access, por ello no se exige suscripción, registro o tarifa de acceso a los usuarios o instituciones. Los usuarios pueden leer, descargar, copiar, distribuir, imprimir y compartir los textos completos inmediatamente después de publicados, se exige no hacer uso comercial de las publicaciones. Para la reproducción parcial o total de los trabajos o contenidos publicados, se exige reconocer los derechos intelectuales de los autores y además, hacer referencia a esta revista. La publicación de artículos se hace sin cargo para los autores. Los trabajos pueden consultarse y descargarse libremente, y de manera gratuita, en extenso en versión digital, desde su enlace Web institucional. Los textos publicados son propiedad intelectual de sus autores. Las ideas, opiniones y conceptos expuestos en los trabajos publicados en la revista representan la opinión de sus autores, por lo tanto, son estos los responsables exclusivos de los mismos.
