Identification of filamentous fungi in internal areas of the university hospital "Antonio Patricio de Alcalá", Venezuela

Authors

  • Daniel Jose Muñoz Liceo Bolivariano "José Silverio González" https://orcid.org/0000-0003-1368-8919
  • Rossianny Rodríguez Universidad Politécnica Territorial del Oeste de Sucre "Closdosbaldo Russian".

Keywords:

Hospital environment, fungi, Aspergillus, Penicillium

Abstract

The presence and proliferation of fungi in the hospital environment can represent a risk factor for the health of patients and their families, since they can cause nosocomial infections. The objective of this study was to identify the different filamentous fungi present in the areas: adult emergency, pediatric emergency, Intensive Care Unit and operating room of the “Antonio Patricio de Alcalá” University Hospital in the city of Cumaná, Sucre state in Venezuela. The study was descriptive, cross-sectional and non-experimental. The count of the colony-forming units per plate of the filamentous fungi was performed in Petri dishes with potato dextrose agar, exposed in the spaces mentioned. Subsequently, the fungal colonies present were isolated and identified. Significant differences were observed in the counts obtained from the different studied sites (p <0.05), adult and pediatric emergency presented the highest counts, followed by the Intensive Care Unit and the Operating Room. No significant differences were observed between the sampling months (p> 0.05). The fungal genera that were isolated in the highest proportion were: Aspergillus (36.3%), Penicillium (28.2%), Fusarium (12.7%) and Cladosporium (9.1%). The most frequently isolated species were: A. niger (14.2%), P. citrinum (12.7%), F. poae (12.7%) and C. herbarum (9.1%). The results obtained showed that several of the fungi identified in the present study should be considered as potential pathogens for patients and other people in the hospital.

Downloads

Download data is not yet available.

Author Biographies

Daniel Jose Muñoz, Liceo Bolivariano "José Silverio González"

Licenciado en Biología. Licenciado en Educación, mención Biología. Coordinación de Ciencias Naturales Liceo Bolivariano "José Silverio González". Cumana, Edo. Sucre,Venezuela.

Rossianny Rodríguez, Universidad Politécnica Territorial del Oeste de Sucre "Closdosbaldo Russian".

Lic. Biologia, M.Sc. en Microbiología Aplicada. Docente Departamento de Biología Aplicada. Laboratorio de Microbiología de los Alimentos. Universidad Politécnica Territorial del Oeste de Sucre "Closdosbaldo Russian". Cumana, Edo. Sucre.

References

1. Izzeddin A, Medina L, Rojas T. Evaluación de bioaerosoles en ambientes de centros de salud de la ciudad de Valencia, Venezuela. Kasmera. 2011; 39(1): 59-67.

2. Gonçalves C, Mota F, Ferreira G, Mendes J, Pereira E, Freitas C, Vieira J, Villarreal J, Nascente P. Airborne fungi in an intensive care unit. Braz J Biol. 2018; 78(2): 265-270.

3. Golah H, Al-Garadi M, Salah M, Baghza N, Al-Mahdi H, Al-Dhorani M, Al-Sharma A. Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen. Int J Microbiol Biotechnol. 2017; 2(4): 166-170.

4. Marcano-Bruzual J. Aislamiento de hongos anemófilos en el ambiente del servicio de emergencia del Hospital “Luis Daniel Beauperthuy” de Cumanacoa, Municipio Montes, estado Sucre. 2013. (Tesis de Grado). Escuela de Ciencias. Universidad de Oriente. Cumaná. (Venezuela). 43 pp.

5. Martínez-Herrera E, Frías-León M, Duarte-Escalante E, Calderón-Ezquerro M, Jiménez-Martínez M, Acosta-Altamirano G, Rivera-Becerril F, Toriello C, Reyes-Montes M. Fungal diversity and Aspergillus species in hospital environments. Ann Agric Environ Med. 2016; 23(2):264-269.

6. Kanamori H, Rutala W, Sickbert-Bennett E, Weber D. Review of fungal outbreaks and infection prevention in healthcare settings during construction and renovation. Clin Infect Dis. 2015; 61(3): 433–444.

7. Rahman F, Irfan M, Fasih N, Jabeen K, Sharif H. Pulmonary scedosporiosis mimicking aspergilloma in an immunocompetent host: A case report and review of the literature. Infection. 2016; 44(1): 127-132.

8. Overberger P, Wadowsky R, Schaper M. Evaluation of airborne particles and fungi during hospital renovation. J Am Ind Hig Assoc. 1995; 56:706-712.

9. Peckham D, Williams K, Wynne S, Denton M, Pollard K, Barton R. Fungal contamination of nebuliser devices used by people with cystic fibrosis. J Cyst Fibros. 2016; 15(1): 74-77.

10. Mukhia-Rakesh K, Shah-Rakesh P, Urhekar A. Incidence of Candida in Air from the Hospital Environment. Int J Adv Microbiol Health Res. 2017; 1(1):29-33.

11. Koutsounas I, Pyleris E. Isolated enteric aspergillosis in a non severely immunocompromised patient. Case report and literature review. Arab J Gastroenterol. 2015; 16(2): 72-75.

12. Silva S, Sepúlveda M, Vásquez R, Teperman J, Rodríguez L, Parra M, Kimmelman G. Compendio de aspectos teóricos, prácticos en el manejo de áreas de contaminación controlada. Chile: Editorial Universitaria. 1984.

13. Riddel R. Permanents stained micological preparations obtained by slide cultura. Micol. 1950; 43: 265-270.

14. Koneman E, Roberta D. Micología. En: Winn W, Allen S, Janda W, Koneman E, Procop G, Schrenckenberger P, Woods G, editores. Diagnóstico Microbiológico. 6ta. ed. Argentina: Editorial Médica Panamericana; 1992. p. 220-222.

15. Pitt J, Samson R. Systematics of Penicillium and Aspergillus- past, present and future. En: Samson R, Pitt J, editores. Moder concept in Penicillium and Aspergillus classification. Londres. 3-13 pp. 1990.

16. Pitt J. Hocking A, Samson R, King A. Methods for the mycological examination of foods. En: Samson R, Pitt J, King A, editores. Modern methods in food mycology. Oxford: ELSEVIER; 1992. P. 365-368.

17. Samson R, Hoekstra E, Frisvad J, Filtenborg O. Introduction to food and airborne fungi. Netherlands: Utrecht : Centraalbureau voor Schimmelcultures; 1995.
18. Rivas E. Estadística general. Venezuela: Editorial de la Biblioteca de la Universidad Central de Venezuela; 1990.

19. Becerra-Torrejón D, Almanza-Salinas G, Flores-Alarcón A, Santa Cruz A. Bacterias en tapas de antisépticos y pinzas de traspaso en carros de curación de emergencias, Hospital Clínico Viedma 2015. Rev Cient Cienc Méd. 2016; 19(1): 17-21.

20. Perelli A, Calzolaio V, González L, Kirchner E, Lamper D, Leonardo S. Presencia de flora fúngica en áreas internas del Hospital “Dr. Adolfo Prince Lara”, Puerto Cabello, Estado Carabobo. Durante el período 2006-2007. VITAE. 2009; 38: 1-10.

21. Martínez-Herrera E, Frías-León M, Duarte-Escalante E, Calderón-Ezquerro M, Jiménez-Martínez M, Acosta-Altamirano G, Rivera-Becerril F, Toriello C, Reyes-Montes M. Fungal diversity and Aspergillus species in hospital environments. Ann Agric Environ Med. 2016; 23(2): 264-269.

22. Ríos-Yuil J, Arenas R, Fernández R, Calderón-Ezquerro M, Rodríguez-Badillo R. Aeromycological study at the intensive care unit of the “Dr. Manuel Gea Gonzalez” General Hospital. Braz J Infect Dis. 2012; 16(5): 432–435.

23. Hao Z, Ao J, Hao F, Yang Rong-Ya R, Zhu H, Zhang J. Epidemiology of opportunistic invasive fungal infections in China: review of literature. Chin Med J (Engl). 2011; 124(13): 1970–1975.

24. Matheus R. Hongos anemófilos en la unidad de cuidado intensivo del Hospital Universitario “Antonio Patricio de Alcalá” Cumana, estado Sucre. (Pregado). Universidad de Oriente; 2012.

25. Pakshir K, Shekarkhar G, Mostagnie S, Sabayan B, Vaghefikia A. Monitoring of air borne fungi in two general hospitals in Shiraz, Southern Iran. Iran J Med Sci 2015; 32(4):240–244.
26. Kiasat N, Fatahinia M, Mahmoudabadi A, Shokri H. Qualitative and Quantitative Assessment of Air borne Fungal Spores in the Hospitals Environment of Ahvaz City (2016). Jundishapur J Microbiol. 2017; 10(10):141-143.

27. Hedayati M, Mohammadpour R. A survey on the mycological contamination of the air and the equipment of operating rooms of 17 hospitals. J Med Facul Guilan Uni Med Sci. 1999; 8(19): 56 -61.

28. Okten S, Asan A. Airborne fungi and bacteria in indoor and outdoor environment of the Pediatric Unit of Edirne Government Hospital. Environ Monit Assess. 2012; 184(3):1739-1745.

29. Ramírez P, Garnacho-Montero J. Invasive aspergillosis in critically ill patients. Rev Iberoam Micol. 2018; 35(4): 210-216.

30. Wierzbicka M, Podsiadlo B, Janczarski M. Invasive pulmonary aspergillosis caused by Aspergillus ochraceus. Pneumonol Alergol Pol. 1997; 65(3-4): 254-260.

31. Boonsarngsuk V, Eksombatchai D, Kanoksil W, Tantrakul V. Peniciliosis endobronquial: presentación de un caso y revisión de la literatura. Arch Bronconeumol. 2015; 51(5): 25-28.

32. Marques do Nascimento J, Queijeiro López A, Andrade-Araújo M, Anhezini-Araujo L, Alves-Silva F. Airborne Fungi in Indoor Hospital Environments. Int J Curr Microbiol App Sci. 2019; 8(1): 2749-2772.

Published

2020-11-26

How to Cite

Muñoz, D. J., & Rodríguez, R. (2020). Identification of filamentous fungi in internal areas of the university hospital "Antonio Patricio de Alcalá&quot;, Venezuela. Revista Venezolana De Salud Pública, 8(2), 48-65. Retrieved from https://revistas.uclave.org/index.php/rvsp/article/view/2975

Issue

Section

Artículos originales