Effect of LED light on the growth and development of vitroplants of four potato varieties (Solanum tuberosun L)
DOI:
https://doi.org/10.5281/zenodo.14431889Keywords:
Solanum tuberosum; luz; crecimiento; micropropagación.Abstract
In in vitro culture, LED light sources have replaced standard luminescent lamps and have acquired an important role in micropropagation. The effect of LED light on the development of four potato varieties in vitro was studied. The test was carried out in the INIA-Lara in vitro culture laboratory, the varieties used were María Bonita, Atlantic Dalinia and Kennebec. These were placed under photoperiod conditions of 8 hours in darkness and 16 hours with artificial LED light (illumination of 150-163 μmol m-2 s-1) and with an average temperature of 23 ± 2 ºC. The design used was completely randomized with 4 treatments with 5 repetitions of 5 bottles for each one. The in vitro characterization was considered by evaluating the morphological characteristics, length of the vitroplant and the root system, the number of leaves and the diameter of the stem. The LED light source is suitable for potato micropropagation, since good growth and development was observed in the evaluated varieties. Regarding the height of the seedlings, the Atlantic variety presented greater development. On the other hand, the length of the roots was greater in the Kennebec and Atlantic varieties while the Dalinia variety presented the lowest values. Kennebec developed a greater number of leaves, while Dalinia. María Bonita and Atlantic developed statistically equivalent values and finally Kennebec and Atlantic produced thicker stems except Dalinia and María Bonita who produced thinner stems.
Downloads
References
Bello-Bello JJ, Martínez-Estrada E, Caamal-Velázquez JH, Morales-Ramos (2016). Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews). Afr J Biotechnol. 15(8): 272-277.
Casierra-Posada F, Peña-Olmos J. (2015(. Modificaciones fotomorfogénicas inducidas por la calidad de la luz en plantas cultivadas. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales. 39(1);84-92.
Chen LL, Zhang K, Gong XC (2020). Effects of different LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro and minituber production after transplanting in the greenhouse. J Integr Agric; 19(1): 108-119.
Di Rienzo JA, Casanoves F, Balzarini MG, González L, Tablada M y Robledo CW. (2017). InfoStat versión 2017, Grupo InfoStat. FCA, Universidad Nacional de Córdoba, Argentina.
Golovatskaya IF, Karnachuk RA. (2015). Role of green light in physiological activity of plants. Russ J Plant Physiol. 62(6):727-740.
Grishchenko Olga V., Eugeny P. Subbotin, Irina V. Gafitskaya, Yulia V. Ereshchagina, Elena V. Burkovskaya, Yulia A. Khrolenko , Valeria P. Grigorchuk , Olga V. Nakonechnaya ,Victor P. Bulgakov , and Yuri N. Kulchin (2022). Growth of Micropropagated Solanum tuberosum L. Plantlets under Artificial Solar Spectrum and Different Mono- and Polychromatic LED Lights. Horticultural Plant Journal, 8 (2): 205–214.
Gupta SD, Agarwal A. (2017). Artificial lighting system for plant growth and development: Chronological Advancement, Working Principles, and Comparative Assessment. En: Dutta S, editor. Light Emitting Diodes for Agriculture. Kharagpur: Springer. p. 1-25.
Jiang L, Wang Z, Jin G, Lu D, Li X. (2019). Responses of Favorita Potato Plantlets Cultured in Vitro under Fluorescent and Light-Emitting Diode (LED) Light Sources. Am J Potato Res. 96: 396-402.
Medeiros de Araújo M, Barboza A, Oliveria-Filho RA, Camara T, Willadino L, Gouveia-Neto A. (2016). The effect of spectral light quality on in vitro culture of sugarcane. Acta Scientiarum. Biological Sciences. 38(2):157-161.
Miler N, Kulus D, Wozny A, Rymarz D, Hajzer M, Wierzbowski K, Nelke R, Szeffs L. (2019). Aplication of wide-spectrum light-emitting diodes in micropropagation of popular ornamental plant species: a study on plant quality and cost reduction. In Vitro Cellular & Developmental Biology-Plant. 55(1):99-108.
Mitchell CA, Sheibani F. (2019). LED advancements for plant-factory artificial lighting. En Kozai T, Niu G, Takagaki M, editores. Plant Factory: An indoor vertical farming system for efficient quality food production. 2 ed. Inglaterra. Elsevier. p. 167-184. 17.
Murashigue, T., Skoog, F.S. (1962). A revised medium for rapid growth and biossays with tobacco tissue culture. Plant Physiology, 15: 173-197.
Paradiso, R., Arena, C., Rouphael, Y., d’Aquino, L., Makris, K., Vitaglione, P., de Pascale, S., (2019). Growth, photosynthetic activity and tuber quality of two potato cultivars in controlled environment as affected by light source. Plant Biosystems, 153: 725–735.
Priyadarshani P, Batra V. (2017). Tissue Culture of Potato (Solanum tuberosum L.): A review. Int J Curr Microbiol Appl Sci. 6(4): 489-495.
Ramírez-Mosqueda MA, Iglesias-Andreu LG, Bautista-Aguilar JR. (2017a). The Effect of Light Quality on Growth and Development of In vitro Plantlet of Stevia rebaudiana Bertoni. Sugar Tech. 19(3):331-336.
Ramírez-Mosqueda Ma, Iglesias-Andreu LG, Luna-Sánchez IJ. (2017b). Light quality affects growth and development of in vitro plantlet of Vanilla planifolia Jacks. South African Journal of Botany. 109(1):288-293.
Sharry SE, Adema M, Abedini W. (2015). Plantas de probeta: Manual para la propagación de plantas por cultivo de tejidos in vitro. 1a ed. La Plata, Argentina: Editorial de la Universidad de La Plata.201 p
Silva Agurto C, Leiva Mora M, Sanchez Ortiz N, del Castillo Bastidas D. (2023). Influencia de las condiciones de iluminación sobre el establecimiento in vitro de yemas de Solanum tuberosum L. var. Cecilia. Revis Bionatura 8 (3) 9-16
Suarez IE. (2020). Cultivo de tejidos vegetales. 1a Ed. Colombia: Fondo Editorial Universidad de Cordova.120p
Yu L, Song C, Sun L, Li L, Xu Z, Tang, C. (2020). Effects of light-emitting diodes on tissue culture plantlets and seedlings of rice (Oryza sativa L). Journal of Integrative Agriculture. 19(7): 1743-1754
Published
How to Cite
Issue
Section
Copyright (c) 2024 Norkys Marilyn, Israel Alvares, Héctor Carrera, Elsy Bastidas, Ericka Porras
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.