Multiplication of arbuscular mycorrhizal fungi isolated from cocoa cultivated soils




AMF genera, inoculum, massive multiplication, Oryza sativa, Zea Mays


The multiplication of arbuscular mycorrhizal fungi (AMF) spores is a limitation for agricultural applications as biofertilizers. In the present study, a massive multiplication of AMF from cocoa farms (Theobroma cacao L.) in the San Martín region, Peru, was carried out to optimize their handling and spore production under controlled environmental conditions. The experiment was conducted under greenhouse conditions in a completely randomized design, considering three sources of AMF inoculum obtained in the provinces of Mariscal Cáceres, Lamas and Rioja, in two periods (110 and 220 days) and two host crops (maize and rice). This material was mixed with one volume of sand and three volumes of agricultural soil, which were placed in crates. At the end of the periods, the population of spores, mycorrhizal colonization, height of the host plant, and the dry weight of the aerial part and root were evaluated. Rice acts as a suitable host and multiplies a greater number of AMF spores. The treatment based on the mycorrhizal inoculum from Mariscal Cáceres multiplied with rice plants at 220 days, presented the highest population of spores. Therefore, it could be used as a methodology for massive propagation of AMF due to its great reproduction capacity.


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Brundrett, M., N. Bougher, B. Dell, T. Grove y N. Malajczuck. 1996. Working with mycorrhizas in forestry and agriculture. Camberra, Australia: ACIAR. 374 p.

Bustamante, A. y W. Zambrano. 2014. Eficiencia de la multiplicación de hongos micorrízicos arbusculares (HMA), nativos en Zea mays L. en condiciones de invernadero. Universidad Nacional “Pedro Ruiz Gallo”, Lambayeque, Perú. (consulta de abril 14, 2022)

Chaiyasen, A., C. Leardwiriyakool, D.D. Douds y S. Lumyong. 2017. Influence of host plants and soil diluents on arbuscular mycorrhizal fungus propagation for on-farm inoculum production using leaf litter compost and agrowastes. Biol. Agric. Hortic. 33: 52-62.

Corazon-Guivin, M.A., A. Cerna-Mendoza, J.C. Guerrero-Abad, A. Vallejos-Tapullima O. Ríos-Ramírez, G. Vallejos-Torres et al. 2020. Paraglomus occidentale, a new arbuscular mycorrhizal fungus from the sources of the Amazon river in Peru, with a key to the Paraglomeromycetes species. Sydowia 72: 85-94.

Corazon-Guivin, M.A., A. Vallejos-Tapullima, A.M. de La Sota-Ricaldi, A. Cerna-Mendoza, J.C. Guerrero-Abad, V.M. Santos et al. 2021a. Acaulospora flava, a new arbuscular mycorrhizal fungus from Coffea arabica and Plukenetia volubilis plantations at the sources of the Amazon river in Peru. J. Appl. Bot. Food Qual. 94: 116-123.

Corazon-Guivin, M.A., A. Cerna-Mendoza, J.C. Guerrero-Abad, A. Vallejos-Tapullima, G.A. Silva y F. Oehl. 2021b. Paraglomus peruvianum sp. novum. In: R. Lebeuf et al. (eds.). Fungal Systematics and Evolution: FUSE 8. Sydowia 74: 193-249.

Corazon-Guivin, M.A., A. Vallejos-Tapullima, A.M. Sota-Ricaldi1, G. Vallejos-Torres, M.E. Ruíz-Sánchez, V.M. Santos et al. 2022. Acaulospora favopapillosa, a new fungus in the Glomeromycetes from a coffee plantation in Peru, with an updated key for the identification of Acaulosporaceae species. J. Appl. Bot. Food Qual. 95: 6-16.

Covacevich, F., K. Hernández, E.M. Crespo, E. Lumini, M.S. Rivero y M.A. Lugo. 2021. Arbuscular mycorrhizal fungi from Argentinean highland puna soils unveiled by propagule multiplication. Plants (Basel, Switzerland) 10(9): 1803.

Daniels, B.A. y H.A. Skipper. 1982. Methods for the recovery and quantitative estimation of propagules from soil. In: N.C. Schenck (ed.). Methods & Principles of Mycorrhizal Research. American Phytopathological Society. St. Paul, MN, USA. pp. 29-35.

Del Águila Parrillo, K.M., G. Vallejos-Torres, L.A. Arévalo y A. Becerra. 2018. Inoculación de consorcios micorrícicos arbusculares en Coffea arabica, variedad Caturra en la región San Martín. Información Tecnológica 29(1): 137-146.

Gaur, A. y A. Adholeya. 2002. Arbuscular-mycorrhizal inoculation of five tropical fodder crops and inoculum production in marginal soil amended with organic matter. Biol. Fertil. Soils 35: 214-218.

Gerdemann, J.W. y T.H. Nicolson. 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc. 46: 235-244.

Guindon, S. y O. Gascuel. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. System Biol. 52: 696-704.

Hernández-Martínez, M., V.M. Cetina-Alcalá, M.C. González-Chávez y C.T. Cervantes-Martínez. 2006. Inoculación micorrízica y su efecto en el crecimiento de dos leguminosas arbóreas. Terra Latinoamericana 24(1): 65-73.

Ijdo, M., S. Cranenbrouck y S. Dealerck. 2010. Methods for large scale production of AM fungi: past, present and future. Mycorrhiza 21: 1-16.

ICO (International Cocoa Organization). 2014. Cocoa Market Update. (consulta de abril 14, 2022)

Kadian, N., K. Yadav, E. Jangra y A. Aggarwal. 2019. Influencia de la planta hospedante y la paja de arroz como sustrato en la multiplicación masiva de hongos micorrízicos arbusculares para aplicaciones agrícolas a gran escala. Int J. Recycl Org. Waste Agricult. 8: 21-26.

Kadian, N., K. Yadav y A. Aggarwal. 2013. Significance of bioinoculants in promoting growth, nutrient uptake and yield of Cyamopsis tetragonoloba (L.) ‘Taub’. Eur J Soil Biol 58: 66-72.

Kadian, N., K. Yadav y A. Aggarwal. 2018. Mass multiplication of arbuscular mycorrhizal fungi associated with some leguminous plants: an ecofriendly approach. Ind. J. Exp. Biol. 56: 258-266.

Klironomos, J.N. 2003. Variation in plant response to native and exotic arbuscular mycorrhizal fungi. Ecology 84: 2292-2301.

Krüger, M., H. Stockinger, C. Krüger y A. Schüßler. 2009. DNAbased species level detection of Glomeromycota: one PCR primer set for all arbuscular mycorrhizal fungi. New Phytol. 183: 212-223

Larkin, M.A., G. Blackshields, N.P. Brown, R. Chenna, P.A.H. McGettigan, F. Valentin et al. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947-2948.

Milne, I., F. Wright., G. Rowe, D.F. Marshall, D. Husmeier y G. McGuire. 2004. Software for automatic identification of recombinant sequences within DNA multiple alignments. Bioinformatics 20: 806-1807.

Moreno-Miranda, C., I. Molina, Z. Miranda, R. Moreno y P. Moreno. 2020. La cadena de valor de cacao en ecuador: una propuesta de estrategias para coadyuvar a la sostenibilidad. Bioagro 32(3): 205-214.

Muthukumar, T. y K. Udaiyan. 2002. Growth and yield of cowpea as influenced by changes in arbuscular mycorrhiza in response to organic manuring. J. Agron. and Crop Sci. 188(2): 123-132.

Phillips, J.M. y D.S. Hayman. 1970. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycororrhizal fungi for rapid assessment to infection. Trans. Brit. Mycol. Soc. 55: 158-161.

Prieto, O., C. Belezaca, W. Mora, E. Vallejo, L. Gutiérrez y L. Pinargote. 2011. Inoculación de Brachiaria decumbens con hongos formadores de micorrizas arbusculares nativos del trópico húmedo ecuatoriano. Ciencia y Tecnología 4(2): 9-18.

Rashid, M.I., L.H. Mujawar, T. Shahzad, T. Almeelbi y I.M. Ismail. 2016. Bacteria and fungi can contribute to nutrients bioavailability and aggregate formation in degraded soils. Microbiol. Res. 183: 26-41.

Ronquist, F., J.P. Huelsenbeck y M. Bayes. 2003. Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.

Sandoval, D. T. 2019. Caracterización de hongos micorrízicos arbusculares nativos predominantes, provenientes de pasturas de la zona de Cuñumbuque, San Martín y propagados en especies forrajeras. Tesis. Universidad Nacional de San Martín, Tarapoto, Perú. 65 p. (consulta de abril 14, 2022)

Selvakumar, G., C.C. Shagol, Y. Kang, B.N. Chung, S.G. Han y T.M. Sa. 2018. Arbuscular mycorrhizal fungi spore propagation using single spore as starter inoculum and a plant host. J. Appl Microbiol. 124(6): 1556-1565.

Schlemper, R.T. y S.L. Stürmer. 2014. On farm production of arbuscular mycorrhizal fungi inoculum using lignocellulosic agrowastes. Mycorrhiza 24(8): 571-580.

Sharma, S., S. Sharma, A. Aggarwal, V. Sharma, M.J. Singh y S. Kaushik. 2017. Multiplication of arbuscular mycorrhizal fungi. Mycorrhizal Fungi 155-173.

Smith, S.E. y D.J. Read. 2008. Mycorrhizal Symbiosis. Academic Press, London.

Utobo, E.B., E. N. Ogbodo y A.C. Nwogbaga. 2011. Techniques for extraction and quantification of arbuscular rmycorrhizal fungi. Libyan Agriculture Research Center Journal International 2(2): 68-78.

Vallejos-Torres, G., T. Sánchez, M.A. García, M. Trigoso y L.A. Arévalo. 2019. Efecto de hongos formadores de micorrizas arbusculares en clones de café (Coffea arabica) variedad Caturra. Acta Agronómica 68(4): 278-284.

Vallejos-Torres, G., R. Ruíz-Valles., C. E. Chappa-Santa María., N. Gaona-Jiménez. y C. Marín. 2022. High genetic diversity in arbuscular mycorrhizal fungi influence cadmium uptake and growth of cocoa plants. Bioagro 34(1): 75-84.

Vu, D., M. Groenewald, M. de Vries, T. Gehrmann, B. Stielow, U. Eberhardt et al. 2019. Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Stud. Mycol. 92: 135-154.

Zangaro, W., R.A. Alves, L.E. Lescano, A.P. Insanely y M.A. Nogueira. 2012. Investment in fine roots and arbuscular mycorrhizal fungi decrease during succession in three Brazilian ecosystems. Biotropica 44 (2): 141-150.



How to Cite

Vallejos-Torres, G., Tenorio-Cercado, M., Gaona-Jimenez, N., Corazon-Guivin, M., Ormeño-Luna, J., Paredes, C., Saavedra, J., Tuesta, J., Tuesta, O., Alguacil, M. M., Becerra, A., & Marín, C. (2022). Multiplication of arbuscular mycorrhizal fungi isolated from cocoa cultivated soils. Bioagro, 34(3), 265-276.