Do storage of Eugenia stipitata seeds affect their germination and efficacy of the tetrazolium viability test?

Authors

DOI:

https://doi.org/10.51372/bioagro361.11

Keywords:

Araçá-boi, calidad de la semilla, especies medicinales, viabilidad

Abstract

Eugenia stipitata is a fruit species from the Amazon that is important in traditional medicine due to its therapeutic properties. Estimates of seed viability in this species are still scarce, and it is necessary to develop rapid tests to determine the quality of the seeds. The aim of this study was to determine the applicability of the tetrazolium test to estimate viability and germination in seeds of E. stipitata with and without storage. The design was completely randomised in a 2 x 2 factorial scheme with 10 replications. The results of the tetrazolium and germination tests show that seeds of E. stipitata lose their viability when stored for six months in water at ambient temperature. Neither small nor large seeds stored for six months in water at ambient temperature are recommended for use with the tetrazolium test. Small, freshly harvested, unstored seeds show a germination potential of 100%. The tetrazolium test is efficient in determining the viability of small and large seeds of Eugenia stipitate with no storage.

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References

Anjos, A.M.G., and I.D.K. Ferraz. 1999. Morfologia, germinação e teor de água das sementes de araçá-boi (Eugenia stipitata ssp. sororia). Acta Amazônica 29(1): 337-348.

Bhéring, M.C., R.F. Silva, E.M. Alvarenga, D.N.F.S. Dias, and M.F. Pena. 1996. Avaliação da viabilidade e do vigor de sementes de feijão de vagem (Phaseolus vulgaris L.) pelo teste de tetrazólio. Boletim Técnico, UFV.Viçosa. 27 p.

Calvi, G.P., F.F. Aud, I.D.K. Ferraz, H.W. Pritchard, and I. Kranner. 2016. Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination. Plant Biology 19(1): 6-13.

Delouche, J.C., T.W. Still, M. Raspet, and M. Lienhard. 1976. O teste de tetrazólio para viabilidade da semente. Brasília: Agiplan, 103p.

França-Neto, J.B., and F.C. Krzyzanowski. 2019. Tetrazolium: an important test for physiological seed quality evaluation. Journal of Seed Science 41(2): 359-366.

França-Neto, J.B., and F.C. Krzyzanowski. 2022. Use of the tetrazolium test for estimating the physiological quality of seeds. Seed Science and Technology 50(1): 31-44.

Gentil, D.F.O., and S.A.N. Ferreira. 1999. Viabilidade e superação da dormência em sementes de araçá-boi (Eugenia stipitata ssp. sororia). Acta Amazônica 29(3): 21-31.

Gomes, P., I. Marquesand, and F. Martins. 2006. Germination of Geonoma brevispatha in laboratory and its relation to the palm spatial distribution in a swamp forest Aquatic Botany 85(4): 16-2.

Mata, D.I., and P. Moreno-Casasola. 2005. Effect of in situ storage, light, and moisture on the germination of two wetland tropical trees. Aquatic Botany 83(1): 206-218.

Magnitskiy, S.V., and G.A. Plaza. 2007. Fisiología de semillas recalcitrantes de árboles tropicales. Agronomía Colombiana 25(1): 96-103.

Menegatti, R.D., A.G. Souza, and V.J. Bianchi. 2019. Estimating genetic divergence between peach rootstock cultivars using multivariate techniques based on characteristics associated with seeds. Genetics and Molecular Research 1(3): 01-10.

Ministério da Agricultura, Pecuária e Abastecimento. 2009. Regras para análise de sementes. Brasília. 399 p. https://www.gov.br/ agricultura/pt-br/assuntos/insumos-agropecuarios/ arquivos-publicacoes-insumos/2946_regras_ analise__sementes.pdf

Moura, M.L.S., E.A. Chagas, O.J. Smiderle, R. Vilaça, P.C. Chagas, E.A. Moura, and E.E. Farias. 2016. Biometric characterization, water absorption curve and vigor on araçá-boi seeds. International Journal of Plant Biology 7(2): 6265.

Paraíso, H.A., D.S. Brandão Junio, R.I.S. Avelar, C.A. Costa, L.S.P. Gomes, and W.M. Nascimento. 2019. Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds. Journal of Seed Science 41(1): 007-012.

R Core Team. 2018. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/

Sáenz Ramírez, L.H., A.M.S. Mendes, A.M. Imakawa, P.T.B. Sampaio, and M.G.G. Melo. 2021. Viability of stored seeds of Himatanthus sucuuba Wood by tetrazolium test. Ciência Florestal 31(1): 333-349.

Silva, M.L., E.A. Chagas, R. Vilaça, O.J. Smiderle, E.A. Moura, and Chagas, P.C. 2016. Osmopriming duration in Araçá-boi seeds germination. Revista Agro@mbiente On-line 10(1):17 - 21.

Silva, J.N., E.A. Alves, M.L.S. Medeiros, G.V.G. Pádua, M.J. Silva, M.H.B.S. Rodrigues et al. 2022. Caracterização morfológica de frutos e sementes em uma população natural de Hymenaea martiana Hayne. Scientia Forestali, 50(4): e3929.

Smiderle, O.J., and A.G. Souza. 2021. Do scarification and seed soaking periods promote maximum vigor in seedlings of Hymenaea courbaril? Journal of Seed Science 43(1): e202143030.

Souza, O.M., O.J. Smiderle, A.G. Souza, E.A. Chagas, P.C. Chagas, C.G. Bacelar Lima, and B.S. Morais. 2017. Influência do tamanho da semente na germinação e vigor de plântulas de populações de camu-camu. Scientia Agropecuaria 8(2): 119-125.

Souza, A.G., O.J. Smiderle, and C.A. Pedrozo. 2019. Long-time storage Pochota fendleri seeds with different packaging. Advances in Horticultural Science 33(3): 327-332.

Published

2024-01-04

How to Cite

da Silva Maia, S., Smiderle, O. J., Souza, A. das G., & Barros Torres, S. (2024). Do storage of Eugenia stipitata seeds affect their germination and efficacy of the tetrazolium viability test?. Bioagro, 36(1), 113-120. https://doi.org/10.51372/bioagro361.11

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Section

Nota Técnica