Molecular characterization of Phytophthora cinnamomi, the cause of root rot in Cherimoya (Annona cherimola L.) on the central coast of Peru
DOI:
https://doi.org/10.51372/bioagro371.4Keywords:
Cox2, EF1-α, Oomycete, soil pathogen, zoosporeAbstract
Cherimoya (Annona cherimola Mill.) is a plant that is cultivated mainly in the inter-Andean valleys of the Lima region, Peru, which present suitable conditions for its production. However, these conditions also favor the development of pathogens that cause the decline and death of cherimoya plants. The objective of the study was to molecularly identify the causal agent of cherimoya root rot. Cherimoya roots with mild and severe symptoms of root rot were collected from the valleys: Huanangui, San Antonio de Cumbe and Huaral, Lima. Seven Phytophthora isolates were obtained on PDA and V8 media with coralloid, petiolate mycelium, coenocytic hyphae with hyphal swellings, obpyriform sporangia, ovoid without papillae and globose chlamydospores. The cytochrome oxidase subunit II (Cox2) and elongation factor alpha 1 (EF1-α) regions were amplified using primers FM75, FM78, EF1AF and EF1AR. The sequences obtained showed a similarity of 99,22 to 99,87 % for Cox2 and 99,04 to 100 % with EF1-α for Phytophthora cinnamomi. The phylogenetic tree showed a topology consistent with the Phytophthora accessions. It is concluded that P. cinnamomi is the cause of root rot, this being the first report on the presence of this pathogen in custard apple plants in Peru.
Downloads
References
Abad, G., I. Burgess, T. Bourret, K. Bensch, S. Cacciola, B. Scanu et al. 2023a. Phytophthora: taxonomic and phylogenetic revision of the genus. Studies in Mycology 106: 259-348.
Abad, Z.G., T.I. Burgess, J. Redford, J.C. Bienapfl, R. Mathew, K. Srivastava y K. Jennings 2023b. IDphy: An international online resource for molecular and morphological identification of Phytophthora. Plant Disease 107(4): 987-998.
Bapat, V.A., U.B. Jagtap, S.B. Ghag y T.R. Ganapathi. 2019. Molecular approaches for the improvement of under-researched tropical fruit trees: jackfruit, guava, and custard apple. International Journal of Fruit Science 20(3): 233-281.
Blair, J.E., D. Coffey, Y. Park, M. Geiser y S. Kang. 2008. A multi-locus phylogeny for Phytophthora utilizing markers derived from complete genome sequences. Fungal Genetics and Biology 45(3): 266-277.
Bregant, C., A. Mulas, G. Rossetto, A. Deidda, L. Maddau, G. Piras y T. Linaldeddu. 2021. Phytophthora mediterranea sp. nov., a new species closely related to Phytophthora cinnamomi from nursery plants of Myrtus communis in Italy. Forests 12: 682.
Burgess, T., J. Scott, K. Mcdougall, M. Stukely, C. Crane, W. Dunstan et al. 2017. Current and projected global distribution of Phytophthora cinnamomi, one of the world’s worst plant pathogens. Global Change Biology 23: 1661-1674.
Buse, T.E., Ch.M. Chong y M.J. Salazar. 2015. Modelo de gestión empresarial asociativo para PROACHIRKO, comunidad de Huanangui, Perú. Industrial Data 18(2): 14-19.
Del Castillo-González, L., S. Soudani, N. De La Cruz-Gómez, J.A. Manzanera y M. Berrocal-Lobo. 2024. An improved method to study Phytophthora cinnamomi Rands zoospores interactions with host. BMC Plant Biology 24(1): 508.
Dos Santos, R., A. Leitao, M. da Silva, A. Fernandes y A. Falqueto. 2016. Evaluation of in vitro inhibition of mycelial growth of Fusarium solani f. sp. piperis by different products in Brazil. African Journal of Microbiology Research 10(47): 1992-1998.
Drenth, A. y B. Sendall. 2004. Isolation of Phytophthora from infected plant tissue and soil, and principles of species identification. In: Drenth and D.I. Guest (ed.). Diversity and management of Phytophthora in Southeast Asia. ACIAR. Canberra, Australia. pp. 94-102.
Durán, A.G., M.T. Gutiérrez, F.J. Mejías, J.M. Molinillo y F.A. Macías. 2021. An overview of the chemical characteristics, bioactivity and achievements regarding the therapeutic usage of acetogenins from Annona cherimola Mill. Molecules (Basel, Switzerland) 26(10): 2926.
Erwin, D.C. y O.K. Ribeiro. 1996. Phytophthora diseases worldwide. The American Phytopathology Society. Saint Paul, Minnesota, USA. 592 p.
Fajardo, S.N., S. Valenzuela, A.F. Dos Santos, M.P. González y E.A. Sanfuentes. 2017. Phytophthora pseudosyringae associated with the mortality of Nothofagus obliqua in a pure stand in central-southern Chile. Forest Pathology 47(6): e12361.
Gastañadui, P., R. Moreno, P. Quiroz-Delgado y W. Apaza-Tapia. 2021. Control of avocado root rot caused by Phytophthora cinnamomi with different Trichoderma strains at Chavimochic Irrigation Project. Peruvian Journal of Agronomy 5(3): 78-86.
Gayoso B. y Ch. Chang. 2017. Annona cherimola Mill. "chirimoya" (Annonaceae), una fruta utilizada como alimento en el Perú prehispánico. Arnaldoa 24(2): 619-634.
Gentile, C., G. Mannino, E. Palazzolo, G. Gianguzzi, A. Perrone, G. Serio y V. Farina. 2020. Pomological, sensorial, nutritional and nutraceutical profile of seven cultivars of cherimoya (Annona cherimola Mill). Foods (Basel, Switzerland) 10(1): 35.
Ghezzi, I. 2011. El análisis composicional en el estudio de la producción y distribución de la cerámica prehispánica. Bulletin de l'Institut français d'études andine 40(1):1-29.
Goker, M., H. Voglmayr, A. Riethmüller y F. Oberwinkler. 2007. How do obligate parasites evolve? A multi-gene phylogenetic analysis of downy mildews. Fungal Genetics and Biology 44(2): 105-122.
Hardham, A.R. y L.M. Blackman. 2018. Phytophthora cinnamomi. Molecular Plant Pathology 19(2): 260-285.
Huarhua, M., J. Flores, R. Acuña y W. Apaza. 2018. Morphological and molecular identification of Phytophthora cinnamomi Rands as causal agent of crown and root rot in Blueberry (Vaccinium corymbosum) in Peru. Peruvian Journal of Agronomy 2(2):14-21.
Kumar, S., P. Chowdappa, V. Krishna y H. Sandhya. 2015. Induction of defense-related proteins and growth promotion in tomato by mixture of Trichoderma harzianum OTPB3 and Bacillus subtilis OTPB1 and Pseudomonas putida OPf1 against Phytophthora infestans. African Journal of Microbiology Research 9(2): 96-110.
Larach, A., X. Besoain y E. Salgado. 2009. Crown and root rot of highbush blueberry caused by Phytophthora cinnamomi and P. citrophthora and cultivar susceptibility. Ciencia e Investigación Agraria 36: 433-442.
Martin, F. y P. Tooley. 2003. Phylogenetic relationships among Phytophthora species inferred from sequence analysis of mitochondrially encoded cytochrome oxidase I and II genes. Mycologia 95(2): 269-284.
Martin, F., Z. Abad, Y. Balci y K. Ivors. 2012. Identification and Detection of Phytophthora: Reviewing Our Progress, Identifying Our Needs. Plant Disease 96(8): 1080-1103.
MINAGRI. Ministerio de Agricultura y Riego. 2019. Anuario estadístico de producción agrícola 2018. Dirección General de Seguimiento y Evaluación de Políticas. Lima, Perú. 370 p.
Mostowfizadeh-Ghalamfarsa, R. y Z. Banihashemi. 2016. Species-specific PCR identification and detection of Phytophthora drechsleri, P. cryptogea and P. erythroseptica. Iranian Journal of Plant Pathology 51(4): 541-553.
Naher, L., M.A. Ali y S. Sheheli. 2016. Effect of Seed Treatment on Seed Borne Fungi of Rice. Progressive Agriculture 27: 48-56.
Nechwatal, J., A. Schlenzig, T. Jung, D.E. Cooke, M. Duncan y W.F. Osswald. 2001. A combination of baiting and PCR techniques for the detection of Phytophthora quercina and P. citricola in soil samples from oak stands. Forest Pathology 31(2): 85-97.
Olson, H., y D. Benson. 2011. Characterization of Phytophthora spp. on floriculture crops in North Carolina. Plant Disease 95(8): 1013-1020.
Quesada-Ocampo, L., C. Parada-Rojas, Z. Hansen, G. Vogel, C. Smart, M. Hausbeck et al. 2023. Phytophthora capsici: Recent progress on fundamental biology and disease management 100 years after its description. Annual Review of Phytopathology 61: 185-208.
Scagel, C.F., J.E. Weiland, R.B. Beck y J.N. Mitchell. 2023. Temperature and fungicide sensitivity in three prevalent Phytophthora species causing Phytophthora root rot in Rhododendron. Plant disease 107(10): 3014-3025.
Scanu, B., G. Hunter, B. Linaldeddu, A. Franceschini, L. Maddau, T. Jung et al. 2014. A taxonomic re-evaluation reveals that Phytophthora cinnamomi and P. cinnamomi var. parvispora are separate species. Forest Pathology 44(1): 1-20.
Scarlett, K., D. Collins, L. Tesoriero, L. Jewell, F. Van Ogtrop y R. Daniel. 2016. Efficacy of chlorine, chlorine dioxide and ultraviolet radiation as disinfectants against plant pathogens in irrigation water. European Journal of Plant Pathology 145: 27-38.
Tamura, K. y M. Nei. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512-526.
Tamura, K., G. Stecher y S. Kumar. 2021. MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38(7): 3022-3027.
Tineo, J. 2019. Caracterización morfológica y análisis de la variabilidad genética de la colección nacional de germoplasma de chirimoyo (Annona cherimola Mill.) del Perú. Instituto Nacional de Innovación Agraria. Lima, Perú. pp. 2.
Wang, T., X. Wang, X. Zhu, Q. He y L. Guo. 2020. A proper PiCAT2 level is critical for sporulation, sporangium function, and pathogenicity of Phytophthora infestans. Molecular Plant Pathology 21(4): 460-474.
Zelaya-Molina, L., M. Ortega y A. Dorrance. 2011. Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis. Biotechnology Letters 33(4): 715-720.
Zentmyer, G., J. Leary, L. Klure y G. Grantham. 1976. Variability in growth of Phytophthora cinnamomi in relation to temperature. Phytopathology 66: 982-986.
Zhou, J., Y. Qi, J. Nie, L. Guo, M. Luo, H. McLellan et al. 2022. A Phytophthora effector promotes homodimerization of host transcription factor StKNOX3 to enhance susceptibility. Journal of Experimental Botany 73(19): 6902-6915.
Zumaila, F., A. Jeevalatha y C.N. Biju. 2023. Genetic diversity, mating type and pathogenicity of two Phytophthora species infecting black pepper in India. 3 Biotech 14(1): 1.
Published
How to Cite
Issue
Section
Copyright (c) 2024 Yuri Calle-Cheje, René Aguilar-Anccota, Robert Rafael-Rutte, José Silupú-Masías, Arturo Morales-Pizarro, Merici Medina-Guerrero
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Rights of the author/s are from the year of publication
This work is under the license:
Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
The opinions expressed by the authors not necesarily reflect the position of the publisher or UCLA. The total or partial reproduction of the texts published in this journal is authorized, as long as the complete source and the electronic address of this journal is cited. Authors have the right to use their articles for any purpose as long as it is done for non-profit purposes. Authors can publish the final version of their work on internet or any other medium, after it has been published in this journal.
Bioagro reserves the right to make textual modifications and technical adjustments to the figures of the manuscripts, in accordance with the style and specifications of the journal.
