Microorganisms in the rhizosphere of symptomatic and asymptomatic plants exposed to HLB in a commercial Citrus orchard in Tamaulipas, Mexico
DOI:
https://doi.org/10.51372/bioagro372.10Keywords:
Citrus, diversity, HLB, metagenomicAbstract
Huanglongbing (HLB) is a devastating disease of citrus characterized by yellow spots on the foliage, leaf chlorosis, small and deformed fruits, premature leaf drop, and tree decline which is caused by Candidatus Liberibacter asiaticus. The state of Tamaulipas in Mexico is one of the main producers of Citrus in the country. Given the arrival of HLB in the region, this work focuses on analyzing the individual rhizosphere from symptomatic and asymptomatic orange, lemon, and grapefruit plants cultivated in an orchard in the state. Microbial profiles were analyzed by using a metagenomic approach and bioinformatic tools. The results show that microbial diversity is different between symptomatic and asymptomatic lemon plants. Particularly, the asymptomatic grapefruit plants show the lowest microbial diversity. In general, all Citrus species show differences in their bacterial population structure, identifying groups related to beneficial bacteria, and differences in microbial diversity, according to the presence of HLB symptoms.
Downloads
References
Biblioteca de Publicaciones Oficiales. 2022. Producción de cítricos en México. Gobierno de México: https://www.gob.mx/publica- ciones/articulos/produccion-de-citricos-en-mexico
Blacutt, A., Ginnan, N., Dang, T., Bodaghi, S., Vidalakis, G., Ruegger, P et al. (2020). An in vitro pipeline for screening and selection of citrus-associated Microbiota with potential anti-“Candidatus Liberibacter asiaticus” properties. Applied and Environmental Microbiology 86(8) 1-18.
Callahan, B.J., P.J. McMurdie, M.J Rosen, A.W. Han, A.J.A. Johnson and S.P. Holmes. 2016. DADA2: High-resolution sample inference from Illumina amplicon data. Nature Method 13: 581-583.
Gao, C., C. Li, Z. Li, Y. Liu, J. Li, J. Guo et al. 2023. Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus”. Frontiers in Plant Science 14:1-13.
Garza-Saldaña, J.J., S. Varela-Fuentes and W. Gómez-Flores. 2017. Métodos para la detección presuntiva de Huanglongbing (HLB) en cítricos CienciaUAT 11(2): 93-104.
Ginnan, N.A., T. Dang, S. Bodaghi, P.M. Ruegger, G. McCollum, G. England. et al. 2020. Disease-induced microbial shifts in citrus indicate microbiome-derived responses to huanglongbing across the disease severity spectrum. Phytobiomes Journal 4(4): 375-387.
Li, B., Y. Wang, T. Hu, D. Qiu, F. Francis, S. Wang and S. Wang. 2022. Root-associated Microbiota response to ecological factors: Role of soil acidity in enhancing citrus tolerance to Huanglongbing. Frontiers in Plant Science 13.
Lombardo, M.F., Y. Zhang, J. Xu, P. Trivedi, P. Zhang, N. Riera et al. 2024. Global citrus root microbiota unravels assembly cues and core members. Frontiers in Microbiology 15: 1-18.
Márquez-Pérez, F.J., J.L. Flores-Sánchez, L. Rodríguez Mejía, J. Márquez Gómez, S.J. Michereff, V. Ancona et al. 2018. Progress and spatial pattern of huanglongbing in Persian lime in Nayarit, Mexico. Revista Bio Ciencias 5(nesp1), e351.
Park, J.W., W.E. Braswell and M. Kunta. 2023. Co-occurrence analysis of citrus root bacterial Microbiota under citrus greening disease. Plants 13(1): 80.
Qureshi, J.A., B.C. Kostyk and P.A. Stansly. 2014. Insecticidal suppression of Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae) vector of huanglongbing pathogens. PloS One 9(12): e112331.
SENACICA. 2022. Análisis de impacto económico ante un posible establecimiento y dispersión del cancro de los cítricos en México en áreas comerciales. https://n9.cl/1f71p
Shahzad, F., C. Chun, A. Schumann and T. Vashisth. 2020. Nutrient uptake in Huanglongbing-affected sweet orange: Transcriptomic and physiological analysis. Journal of the American Society for Horticultural Science 145(6): 349-362.
SIAP. (Servicio de Información Agroalimentaria y Pesquera) 2018. Infografía: Huanglongbing, Análisis Epidomiológico Tamaulipas. https://prod. senasica.gob.mx/SIRVEF/ContenidoPublico/HLB/Infografias/Tamaulipas.pdf
Trivedi, P., Z. He, J.D. Van Nostrand, G. Albrigo, J. Zhou and N. Wang. 2012. Huanglongbing alters the structure and functional diversity of microbial communities associated with citrus rhizosphere. The ISME Journal 6(2): 363-383.
Xi, M., E. Deyett, J.E. Stajich, A. El-Kereamy, M.C. Roper and P.E. Rolshausen, 2023. Microbiome diversity, composition and assembly in a California citrus orchard. Frontiers in Microbiology 14: 1-11.
Yan, H., B. Zhou, B. Jiang, Y. Lv, M.D. Moniruzzaman, G. Zhong and Y. Zhong 2021. Comparative analysis of bacterial and fungal endophytes responses to Candidatus Liberibacter asiaticus infection in leaf midribs of Citrus reticulata cv. Shatangju. Physiological and Molecular Plant Pathology 113(101590): 1-10.
Published
How to Cite
Issue
Section
Copyright (c) 2025 Jesús G. García Olivares, Homar R. Gill Langarica, José A. Narváez Zapata

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Rights of the author/s are from the year of publication
This work is under the license:
Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
The opinions expressed by the authors not necesarily reflect the position of the publisher or UCLA. The total or partial reproduction of the texts published in this journal is authorized, as long as the complete source and the electronic address of this journal is cited. Authors have the right to use their articles for any purpose as long as it is done for non-profit purposes. Authors can publish the final version of their work on internet or any other medium, after it has been published in this journal.
Bioagro reserves the right to make textual modifications and technical adjustments to the figures of the manuscripts, in accordance with the style and specifications of the journal.