Bacterial diversity in sugarcane across different cultivation periods in southern Tamaulipas, Mexico
DOI:
https://doi.org/10.51372/bioagro373.2Keywords:
Agricultural soil, microbiota, rhizosphereAbstract
This study analyzed the rhizosphere bacterial communities in sugarcane fields subjected to different durations of agronomic management: recent (5 years) and long-term (over 50 years). The objective was to evaluate the diversity of rhizosphere bacteria using 16S rRNA gene sequencing and assess their correlation with soil management practices. Results showed distinct bacterial compositions between recently and long-term managed fields. Significant associations were observed between bacterial species and soil conditions characterized by high electrical conductivity (EC), elevated concentrations of Ca²⁺, K⁺, Fe²⁺/Fe³⁺, and Mg²⁺, and low CO₃²⁻ levels. Dominant species included Bacillus megaterium, Bacillus subtilis, and Bacillus simplex, representing the phyla Actinobacteria, Proteobacteria, and Firmicutes, respectively. An analysis of the outlying mean index explained 76.2 % of the total variation, highlighting key soil factors. The first axis (44.7 %) correlated with EC and organic matter content (OM), while the second axis (31.5 %) was associated with extractable phosphorus (P) and potassium (K) levels. These findings underscore the influence of soil physicochemical properties-such as EC, OM, and nutrient availability-on rhizosphere bacterial diversity and composition. These variables modify the soil microenvironment, favoring certain bacterial groups over others and directly impacting soil fertility and agricultural productivity. This study provides insights into the interaction between rhizosphere bacteria and soil management practices. Understanding microbial dynamics in long-term managed systems paves the way for strategies that improve soil health and crop productivity in intensive agricultural systems.
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Adams, A.J., J.P. LaBonte, K. Ball, K.L. Richards-Hrdlicka, M.A. Toothman and C.J. Briggs. 2015. DNA extraction method affects the detection of a fungal pathogen in formalin-fixed specimens using qPCR. PLoS One 10(8): e0135389.
Aguilar-Rivera, N., L.A. Olvera and G. Galindo. 2013. Evaluación de aptitud de tierras al cultivo de caña de azúcar en la Huasteca Potosina, México, por técnicas geomáticas. Revista de Geografía Norte Grande 55: 141-156.
Ali, A., A. Rasheed, A. Siddiqui, N. Naseer, S. Wasim and W. Akhtar. 2015. Non-parametric test for ordered medians: the Jonckheere Terpstra Test. International Journal of Statistics in Medical Research 4(2): 203-207.
Arunrat, N., C. Sansupa, P. Kongsurakan, S. Sereenonchai and R. Hatano. 2022. Soil microbial diversity and community composition in rice-fish co-culture and rice monoculture farming systems. Biology 11(8): 1242.
Bhattacharyya, P.N. and D.K. Jha. 2012. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology 28: 1327-1350.
Cabrera, J.A. and R. Zuaznábar. 2010. Impacto sobre el ambiente del monocultivo de la caña de azúcar con el uso de la quema para la cosecha y la fertilización nitrogenada. I. Balance del carbono. Cultivos Tropicales 31(1): 5-13.
Ciccazzo, S., A. Esposito, E. Rolli, S. Zerbe, D. Daffonchio and L. Brusetti. 2014. Different pioneer plant species select specific rhizosphere bacterial communities in a high mountain environment. Springer Plus 3: 391.
Cruz, D.R., M.A. Silva, A.S. Nascente, M.C. Filippi and E. Ferreira. 2023. Use of multifunctional microorganisms in corn crop. Revista Caatinga 36(2): 349-361.
Cumpa-Velásquez, L.M., J.I. Moriconi, D.P. Dip, L.N. Castagno, M.L. Puig, S.J. Maiale and M.J. Estrella. 2021. Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis. Applied Soil Ecology 168: 104125.
Dolédec, S., D. Chessel and C. Gimaret-Carpentier. 2000. Niche separation in community analysis: a new method. Ecology 81(10): 2914-2927.
Ducousso-Détrez, A., R. Raveau, J. Fontaine, M. Hijri and A. Lounès-Hadj Sahraoui. 2022. Glomerales dominate arbuscular mycorrhizal fungal communities associated with spontaneous plants in phosphate-rich soils of former rock phosphate mining sites. Microorganisms 10(12): 2406.
Elbagory, M. 2023. Reducing the adverse effects of salt stress by utilizing compost tea and effective microorganisms to enhance the growth and yield of wheat (Triticum aestivum L.) plants. Agronomy 13(3): 823.
Galperin, M.Y. 2013. Genome diversity of spore-forming Firmicutes. Microbiology Spectrum 1(2): TBS-0015-2012.
Garrity, G.M., D.J. Brenner, N.R. Krieg and J.T. Staley. 2005. Bergey’s Manual of Systematic Bacteriology, 2nd ed. Springer, New York.
He, C., K. Li, C. Wen, J. Li, P. Fan, Y. Ruan and Z. Jia. 2023. Changes in physicochemical properties and bacterial communities of tropical soil in China under different soil utilization types. Agronomy 13(7): 1897.
House, C.H., M. Pellegrini and S.T. Fitz-Gibbon. 2015. Genome-wide gene order distances support clustering the gram-positive bacteria. Frontiers in Microbiology 6: 785.
IUSS Working Group WRB. 2015. World Reference Base for Soil Resources 2014, update 2015: International soil classification system for naming soils and creating legends for soil maps. FAO, Rome.
Jongenburger, I., M.W. Reij, E.P.J. Boer, L.G.M. Gorris and M.H. Zwietering. 2010. Factors influencing the accuracy of the plating method used to enumerate low numbers of viable micro-organisms in food. International Journal of Food Microbiology 143(1-2): 32-40.
Juma, E.O.A., D.M. Musyimi and G. Opande. 2018. Enumeration and identification of rhizospheric microorganisms of sugarcane variety CO 421 in Kibos, Kisumu County, Kenya. Journal of Asian Scientific Research 8(3): 113-127.
Kumar, A. and R. Chandra. 2020. Ligninolytic enzymes and their mechanisms for degradation of lignocellulosic waste in the environment. Heliyon 6(2): e02310.
Nyenda, T., T. Musungwa, T. Piyo, P. Kowe, J. Muvengwi and E.T.F. Witkowski. 2023. Edaphic and topographic gradients have differential influence on woody species assemblages on ultramafic and non-ultramafic soils in an African Savanna. Plant and Soil 493: 249-266.
Palansooriya, K.N., L. Shi, B. Sarkar, S. Parikh, M.K. Sang, S.R. Lee and Y.S. Ok. 2022. Effect of LDPE microplastics on chemical properties and microbial communities in soil. Soil Use and Management 38(3): 1481-1492.
Pirhadi, M., N. Enayatizamir, H. Motamedi and K. Sorkheh. 2018. Impact of soil salinity on diversity and community of sugarcane endophytic plant growth promoting bacteria (Saccharum officinarum L. Var. CP48). Applied Ecology and Environmental Research 16: 725-739.
Pisa, G., G. Magnani, H. Weber, E.M. Souza, H. Faoro, R.A. Monteiro, E. Daros, V. Baura, J.P. Bespalhok, F.O. Pedrosa and L.M. Cruz. 2011. Diversity of 16S rRNA genes from bacteria of sugarcane rhizosphere soil. Brazilian Journal of Medical and Biological Research 44(12): 1215-1221.
Quecine, M.C., W.L. Araújo, P.B. Rossetto, A. Ferreira, S. Tsui, P.T. Lacava and A.A. Pizzirani-Kleiner. 2012. Sugarcane growth promotion by the endophytic bacterium Pantoeaagglomerans 33.1. Applied and Environmental Microbiology 78(21): 7511-7518.
Rathinasabapathi, B., X. Liu, Y. Cao and L.Q. Ma. 2018. Phosphate-solubilizing Pseudomonads for improving crop plant nutrition and agricultural productivity. In: Crop Improvement Through Microbial Biotechnology, pp. 363-372. Elsevier.
Roesch, L.F.W., F.A.O. Camargo, F.M. Bento and E.W. Triplett. 2007. Biodiversity of diazotrophic bacteria within the soil, root and stem of field-grown maize. Plant and Soil 302: 91-104.
Rosa, P.A.L., E.S. Mortinho, A. Jalal, F.S. Galindo, S. Buzetti, G.C. Fernandes and M.C.M. Filho. 2020. Inoculación con bacterias promotoras del crecimiento asociadas a la reducción de la fertilización fosfatada en caña de azúcar. Fronteras en las Ciencias Ambientales 8: 32.
Sanguin, H., B. Remenant, A. Dechesne, J. Thioulouse, T.M. Vogel, X. Nesme and G.L. Grundmann. 2006. Potential of a 16S rRNA-based taxonomic microarray for analyzing the rhizosphere effects of maize on Agrobacterium spp. and bacterial communities. Applied and Environmental Microbiology 72(6): 4302-4312.
Visi, D.K., N. D'Souza, B.G. Ayre, C.L. Webber and M.S. Allen. 2013. Investigation of the bacterial retting community of kenaf (Hibiscus cannabinus) under different conditions using next-generation semiconductor sequencing. Journal of Industrial Microbiology & Biotechnology 40: 465-475.
Wakgari, T., K. Kibret, B. Bedadi, M. Temesgen and T. Erikossa. 2018. Long-term effects of cultivation on physicochemical properties of soils at Metahara sugar estate. American-Eurasian Journal of Agricultural Research 18: 246-257.
Wang, H.B., Z.X. Zhang, H.B. Li, C.X. Fang, A.J. Zhang and W.X. Li. 2011. Characterization of metaproteomics in crop rhizospheric soil. Journal of Proteome Research 10(3): 932-940.
Yaghoubi Khanghahi, M., S. Strafella, I. Allegretta and C. Crecchio. 2021. Isolation of bacteria with potential plant-promoting traits and optimization of their growth conditions. Current Microbiology 78: 464-478.
Zhang, J., S. Luo, Z. Yao, J. Zhang, Y. Chen, Y. Sun and C. Tian. 2022. Effect of different types of continuous cropping on microbial communities and physicochemical properties of black soils. Diversity 14(11): 954.
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Copyright (c) 2025 Martín A. Reyes Lara, Juan Flores Gracia, Crystian S. Venegas Barrera, Homar R. Gill Langarica, Jesús D. Quiroz Velásquez, Jesús G. García Olivares
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