Physical and chemical properties of the mulberry grapes (Rubus floribundus Kunth) in North Santander, Colombia
DOI:
https://doi.org/10.51372/bioagro382.8Keywords:
BBCH scale, maturity indexAbstract
Rubus fruits are prized in fresh and processed products due to their high nutritional value, aroma, and colour. This study aimed to evaluate the variation in the physicochemical properties of blackberries (Rubus floribundus Kunth) during ripening in Norte de Santander, Colombia. Fruits were harvested according to their phenological stage (Ff) during ripening (Ff83, Ff85 and Ff87) according to the BBCH scale. Physical and chemical parameters were determined based on Ff and the measurement time (Tm). These were determined according to Ff and measurement time (Tm). A 3x6 factorial design was employed, incorporating three levels of Ff and six Tm (weeks). The results showed significant main effects (p≤0.001) and interactions (p≤0.001) between phenological phases (Ff) and measurement times (Tm). Significant increases (p≤0.001) were observed during ripening according to Ff in equatorial diameter (DE), mass (M), pH and total soluble solids (SST), with the highest values recorded for Ff87 (12,77 mm; 4,03 g; 2,78; 8,81 °Brix, respectively). There was also a significant, high, positive linear correlation (r=0.808; p≤0.001) between DE and M. The maturity index (IM) and SST showed significant differences (p≤0.05) according to Ff, with the highest values recorded as the fruit ripened; while the total titratable acidity (ATT) recorded a significant decrease (p≤0.05) according to Ff, with the lowest value recorded for Ff87 (1.24 % malic ác.). These findings provide valuable information to help determine the optimal harvest time and establish quality standards for producers and the processing industry alike.
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References
AGRONET (Red de Información y Comunicación del Sector Agropecuario Colombiano). 2025. Resultado de la evaluación agrícola municipal del año 2017. Bogotá. (consulta de agosto 16, 2025).
Al-Saif, A.M., M. Abdel-Sattar, D.H. Eshra, L. Sas-Paszt y M.A. Mattar. 2022. Predicting the chemical attributes of fresh citrus fruits using artificial neural network and linear regression models. Horticulturae 8(11): 1016.
AOAC (Association of Official Analytical Chemists). 1995. Official Methods of Analysis.16th ed. AOAC. International. Washington, DC, USA.
AOAC (Association of Official Analytical Chemists). 2005. Official Methods of Analysis.18th ed. AOAC. International. Washington, DC, USA.
Canales, E., R.K. Gallardo, M. Iorizzo, P. Munoz, L.F. Ferrao, C. Luby et al. 2024. Willingness to pay for blueberries: Sensory attributes, fruit quality traits, and consumer’s characteristics. HortScience 59(8): 1207-1218.
Cancino-Escalante, G.O., L.R. Sánchez-Montaño, E. Quevedo-García y C. Díaz-Carvajal. 2011. Caracterización fenotípica de accesiones de especies de Rubus L. de los municipios de Pamplona y Chitagá, región Nororiental de Colombia. Universitas Scientiarum 16(3): 219-233.
Castro, R.I., C. Vásquez-Rojas, M.G.I. Cortiella, C. Parra-Palma, P. Ramos y L. Morales-Quintana. 2023. Evolution of the volatile organic compounds, phenols and antioxidant capacity during fruit ripening and development of Rubus ulmifolius Schott fruits. Horticulturae 9(1): 13.
Chen, J.Y., J. Du, M.L. Li y C.M. Li. 2020. Degradation kinetics and pathways of red raspberry anthocyanins in model and juice systems and their correlation with color and antioxidant changes during storage. LWT 128: 109448.
Cosme, F., T. Pinto, A. Aires, M.C. Morais, E. Bacelar, R. Anjos et al. 2022. Red fruits composition and their health benefits-A review. Foods 11(5): 644.
de Souza, V.R., P.A.P. Pereira, T.L.T. da Silva, L.C. de Oliveira Lima y R. Pio, F. Queiroz. 2014. Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits. Food Chemistry 156: 362-368.
Enz, M. y N. Dachler. 1998. Compendio para la identificación de los estadios fenológicos de especies mono- y dicotiledóneas cultivadas. Escala BBCH extendida. Limburgerhof.
Eyduran, S.P., S. Ercisli, G. Ilhan, N. Ersoy, G. Ozkan, M.R., Bozhuyuk et al. 2024. Characterization of wild red raspberry ecotypes in Northern Anatolia: Insights into sensory, biochemical and antioxidant properties. Folia Horticulturae 36(2): 323-335.
FAO (Food and Agriculture Organization of the United Nations). 2023. Food loss and waste database. (consulta de julio 16, 2025).
Franco, G. y J. Bernal. 2020. Tecnología para el Cultivo de la Mora (Rubus glaucus Benth.). Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA). Mosquera.
Fuentealba, C., F. Álvarez, E. Ponce, S. Veas, M. Salazar, D. Romero et al. 2024. Differences in primary metabolism related to quality of raspberry (Rubus idaeus L.) fruit under open field and protected soilless culture growing conditions. Frontiers in Plant Science 14: 1324066.
Galli, V., M.T. Sánchez-Ballesta, A. El-kereamy, R.A. Ayub y W. Jia. 2021. Editorial: Hormonal regulation of non-climacteric fruit development and maturation. Frontiers in Plant Science 12: 690691.
Guerrero-Álvarez, G.E., S, Muñoz-Arias y G.A. Cifuentes-Colorado. 2023. Vitamin C content, anthocyanins and antioxidant capacity of fruits of Rubus glaucus Benth. (mora de Castilla) with and without prickles grown in Risaralda, Colombia. Revista Brasileira de Fruticultura 45: e-509.
Guevara-Terán, M., E. Tejera, W. Vásquez-Castillo, C. Santos-Buelga, A.M. González-Paramás y J.M. Álvarez-Suarez. 2024. Influence of altitudes and development stages on the chemical composition and antioxidant capacity of Andean blackberries (Rubus glaucus Benth). Frontiers in Nutrition 11: 1501889.
Horvitz, S., M. Urbano, C. Arroqui y P. Vírseda. 2024. Low gaseous ozone doses as an elicitor for health-promoting compounds in Andean blackberries (Rubus glaucus Benth). Scientia Horticulturae 336: 113439.
Icontec (Instituto Colombiano de Normas Técnicas y Certificación). 1997. Frutas frescas. Mora de Castilla, especificaciones - NTC 4106. Instituto Colombiano de Normas Técnicas. Bogotá.
Igac (Instituto Geográfico Agustín Codazzi). 2011. Estudio General de Suelos y Zonificación de Tierras del Departamento de Norte de Santander. Bogotá. (consulta de agosto 16, 2025).
La Spada, P., E. Dominguez, A. Continella, A. Heredia y A. Gentile. 2024. Factors influencing fruit cracking: an environmental and agronomic perspective. Frontiers in Plant Science 15: 1343452.
Lápiz-Culqui, Y.K., J.B. Meléndez-Mori, J.J. Tejada-Alvarado, D. Cortez, E. Huaman, V.M.N. Zarantes y M. Oliva. 2024. Study of the physicochemical characteristics, antimicrobial activity, and in vitro multiplication of wild blackberry species from the Peruvian highlands. Scientific Reports 14(1): 3863.
Mikulic-Petkovsek, M., D. Koron, Z. Zorenc y R. Veberic. 2017. ¿Do optimally ripe blackberries contain the highest levels of metabolites?. Food Chemistry 215: 41-49.
Mikulic-Petkovsek, M., R. Veberic, M. Hudina, Z. Zorenc, D. Koron y M. Senica. 2021. Fruit quality characteristics and biochemical composition of fully ripe blackberries harvested at different times. Foods 10(7): 1581.
Mikulic-Petkovsek, M., V. Schmitzer, A. Slatnar, F. Stampar y R. Veberic. 2012. Composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species. Journal of Food Science 77(10): 1064-1070.
Moreno-Medina, B.L., F. Casierra-Posada y J, Cutler. 2018. Phytochemical composition and potential use of Rubus species. Gesunde Pflanzen 70: 65-74.
Munsell, A.H. 2022. A Color Notation: A measured color system, based on the three qualities Hue, Value and Chrom. DigiCat. UK.
Nisar, N., S.M. Wani, I. Bashir, I.A. Zargar, S. Musrtafa, Bhat, Murtaza et al. 2025. Changes in bioactive and physicochemical composition of Rubus during three developmental stages. Plant Foods for Human Nutrition 80: 130.
Onethird. 2022. The ultimate guide to fresh produce shelf life prediction, pp.1-17. (consulta de agosto 16, 2025).
Quevedo-García, E, A.F. González-Pedraza y A.C. Díaz-Rodríguez. 2025b. Fenología, rendimiento y calidad del fruto de mora uva (Rubus floribundus Kunth) en Pamplona, Norte de Santander, Colombia. Revista de Investigación Agraria y Ambiental 16(1): 11-31.
Quevedo-García, E, G.O. Cancino-Escalante y L.R. Sánchez-Montaño. 2025a. Flora arvense asociada al cultivo de la mora (Rubus glaucus Benth.) en los municipios Pamplona y Chitagá, Norte de Santander, Colombia. Tropical and Subtropical Agroecosystems 28(2): 093.
Quispe-Sánchez, L., L.M. Mena-Chacón, E. Hernández-Díaz, R. Siche, I. Yoplac, R. Chuquilín-Goicochea et al. 2025. Physicochemical, functional, and sensory properties of berries at different maturity stages. Applied Food Research 5(2): 101265.
Ramírez, F. 2023. Latin American Blackberries Biology. Mora de Castilla (Rubus glaucus Benth.). Springer Nature. Cham.
Samaniego, I., B. Brito, W. Viera, A. Cabrera, W. Llerena, T. Kannangara et al. 2020. Influence of the maturity stage on the phytochemical composition and the antioxidant activity of four andean blackberry cultivars (Rubus glaucus Benth) from Ecuador. Plants 9(8): 1027.
Sánchez-Betancourt, E., M.C. García-Muñoz, J. Argüelles-Cárdenas, V. Franco-Flórez y V. Núñez-Zarantes. 2020. Fruit quality attributes of ten colombian blackberry (Rubus glaucus Benth) genotypes. Agronomía Colombiana 38(1): 9-18.
Schmidt, M. y E. Felsche. 2023. The effect of climate change on crop yield anomaly in Europe. Climate Resilience and Sustainability 3(1): e61.
Seleiman, M.F., N. Al-Suhaibani, N. Ali, M. Akmal, M. Alotaibi, Y. Refay et al. 2021. Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants 10(2): 259.
Shah, H.M.S., Z. Singh, J. Kaur, M.U. Hasan, A. Woodward y E. Afrifa-Yamoah. 2023. Trends in maintaining postharvest freshness and quality of Rubus berries. Comprehensive Reviews in Food Science and Food Safety 22(6): 4600-4643.
SIB (Sistema de Información de la Biodiversidad de Colombia). 2025. https://n9.cl/0mze9q (consulta de agosto 10, 2025).
Simkova, K., R. Veberic, M. Hudina, M.C. Grohar, M. Pelacci, T. Smrke et al. 2024. Non-destructive and destructive physical measurements as indicators of sugar and organic acid contents in strawberry fruit during ripening. Scientia Horticulturae 327: 112843.
Sinha, S.R., A. Singha, M. Faruquee, Md. A. Sayem Jiku, Md. A. Rahaman, Md. A. Alam y M. Abdul Kader. 2019. Postharvest assessment of fruit quality and shelf life of two elite tomato varieties cultivated in Bangladesh. Bulletin of the National Research Centre 43(185).
Skrovankova, S., D. Sumczynski, J. Mlcek, T. Jurikova y J. Sochor. 2015. Bioactive compounds and antioxidant activity in different types of berries. International Journal of Molecular Sciences 16: 24673-706.
Titiricã, I., I.A. Roman, C. Nicola, M. Sturzeanu, E. Iurea, M. Botu et al. 2023. The main morphological characteristics and chemical components of fruits and the possibilities of their improvement in raspberry breeding. Horticulturae 9(1): 50.
Veberic, R., M. Hudina, Z. Zorenc, D. Koron y M. Senica. 2021. Fruit quality characteristics and biochemical composition of fully ripe blackberries harvested at different times. Foods 10(7): 1581.
Wu, Y., C. Zhang, Z. Huang, L. Lyu, J. Li, W. Li y W. Wu. 2021. The color difference of rubus fruits is closely related to the composition of flavonoids including anthocyanins. LWT 149: 111825.
Zhang, X., J.K.C. Ahuja y B.M. Burton-Freeman. 2019. Characterization of the nutrient profile of processed red raspberries for use in nutrition labeling and promoting healthy food choices. Nutrition and Healthy Aging 5(3): 225-236.
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