In vitro inhibition of Fusarium spp. isolated from bean crop using Argemone ochroleuca extract
DOI:
https://doi.org/10.51372/bioagro362.12Keywords:
Papaveraceae, plant extract, root rotAbstract
Species of the genus Fusarium are the main cause of root rot in bean crops, resulting in yield losses of up to 50%. Synthetic fungicides are the primary tools for their control; however, they cause environmental damage. The objective of this study was to evaluate the effect of extracts from A. ochroleuca on Fusarium spp. under in vitro conditions. During the spring-summer 2022 growing season, plants exhibiting symptoms of wilting and vascular browning were collected. The isolated fungi were purified through monosporic cultures and hyphal tip techniques, and their morphological characteristics were characterized. To determine growth inhibition, a poisoned medium methodology was employed, evaluating two concentrations of extracts (13% and 23%) in both ethanol and aqueous mediums. The obtained isolates corresponded to the morphological characteristics of Fusarium. The extract volumes that showed 100% inhibition were the 23% concentration in both ethanol and aqueous mediums. The 13% ethanol extract exhibited inhibition ranging from 29% to 62% between the first and tenth day of incubation. The 13% aqueous extract displayed inhibition ranging from 25% to 43%. The Argemone ochroleuca extract demonstrates antifungal properties against Fusarium spp. isolated from beans.
Downloads
References
Benchimol‐Reis, L.L., C.J. Bueno, S.A. Carbonell and A.F. Chiorato. 2023. Fusarium wilt–common bean pathosystem: Pathogen variability and genetic control. Crop Science 63(5): 2609-2622.
Brahmachari, G., D. Gorai and R. Roy. 2013. Argemone mexicana: chemical and pharmacological aspects. Revista Brasileira de Farmacognosia 23: 559-567.
Cota-Barreras, C.I., R.S. García-Estrada, J. León-Félix, V. Valenzuela-Herrera, G.A. Mora-Romero, K.Y. Leyva-Madrigal and J.M. Tovar-Pedraza. 2023. Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico. Tropical Plant Pathology preprint
de Carvalho Paulino, J.F., C.P. de Almeida, C.C.F. Barbosa, G. de Moraes, C. Gonçalves, C.J. Bueno and L.L. Benchimol-Reis. 2022. Molecular and pathogenicity characterization of Fusarium oxysporum species complex associated with Fusarium wilt of common bean in Brazil. Tropical Plant Pathology 47(4): 485-494.
de Chaves, M.A., P. Reginatto, B.S. da Costa, R.I. de Paschoal, M.L. Teixeira and A.M. Fuentefria. 2022. Fungicide resistance in Fusarium graminearum species complex. Current Microbiology 79(2): 62-71.
Gahukar, R.T. 2016. Plant-derived products in crop protection: effects of various application methods on pests and diseases. Phytoparasitica 44(3): 379-391.
Guido-Mora, A.Z., M. Blanco-Meneses, M.D.M. Granados-Montero and M. Viñas-Meneses. 2021. Identificación de Fusarium en granos de frijol negro (Phaseolus vulgaris l.) en Costa Rica. Agronomía Costarricense 45(2): 57-69.
Hernández-Ruiz, J., J. Bernal, J.E. Ruiz-Nieto, J. Gonzales-Castañeda and A.I. Mireles-Arriaga. 2020. Argemone ochroleuca: (Papaveraceae), alkaloid potential source for agricultural and medicinal uses. Tropical and subtropical agroecosystems 23(2): 1-13.
Juárez-García, R.A., D. Sanzón-Gómez, L.F. Ramírez-Santoyo, J.E. Ruiz-Nieto, J.P. González and J. Hernández-Ruíz. 2021. Áreas geográficas susceptibles a Fusarium oxysporum en el cultivo de fresa en Guanajuato, México. Bioagro 33(1): 51-58.
Juárez-García, R.A., D. Sanzón-Gómez, L.F. Ramírez-Santoyo, J.E. Ruiz-Nieto and J. Hernández-Ruíz. 2020. Inhibición del crecimiento in vitro de Fusarium oxysporum Schltdl., con extracto de Argemone ochroleuca Sweet (Papaveraceae). Acta Agrícola y Pecuaria 6(1): 1-8.
Li, C., S. Fan, Y. Zhang, X. Zhang, J. Luo and C. Liu. 2022. Toxicity, bioactivity of triazole fungicide metconazole and its effect on mycotoxin production by Fusarium verticillioides: New perspective from an enantiomeric level. Science of The Total Environment 828: 154-432.
Mariscal-Amaro, L.A, A. Rivera-Yerena, P.A. Dávalos-González and D. Ávila-Martínez. 2017. Situación actual de hongos asociados a la secadera de la fresa (Fragaria×ananassa duch.) en Guanajuato, México. Agrociencia 51(6): 673-681.
Martínez-Garnica, M., F. Nieto-Munoz, S. Hernandez-Delgado and N. Mayek-Perez. 2014. Pathogenic and genetic characterization of Mexican isolates of Fusarium solani f. sp phaseoli (Burk.) Snyd. & Hans. Revista de la Facultad de Agronomia de la Universidad del Zulia 31: 539-557.
Montiel-González, L., F. González-Flores, B. Sánchez-García, S. Guzmán-Rivera, F. Gámez-Vázquez, J. Acosta-Gallegos et al. 2005. Especies de Fusarium presentes en raíces de frijol (Phaseolus vulgaris L.) con daños de pudrición, en cinco estados del centro de México. Revista Mexicana de Fitopatología 23(1): 1-7.
Moustafa, M.F., S.A. Alamri, T.H. Taha and S.A. Alrumman. 2013. In vitro antifungal activity of Argemone ochroleuca Sweet latex against some pathogenic fungi. African Journal of Biotechnology 12(10): 1132-1137.
Nelson, P.E., T.A. Toussoun and W.F.O. Marasas. 1983. Fusarium Species: An Illustrated Manual for Identification. The Pennsylvania State University Press. University Park and London, pp: 142-150. http://agris.fao.org/agris-search/search.do? recordID=US19850076353
Rangel-Castillo, A.E., E. Valadez-Moctezuma and H. Lozoya-Saldaña. 2017. Caracterización molecular y patogénesis de Fusarium asociado al amarillamiento del trigo. Revista Fitotecnia Mexicana 40(4): 439-450.
Rangel-Rodriguez, L.S., A. Perez-Silva, M.D.J. Zavala-Guevara, A.G. Hernandez-Juarez and Hernandez-Ruiz. 2023. Aislamiento e identificación de Fusarium oxysporum obtenido de cultivos de Solanum lycopersicum en Irapuato, Guanajuato. Jóvenes en la ciencia 21: 1-4.
Rizvi, R., I. Mahmood, S.A. Tiyagi and Z. Khan. 2012. Effect of some botanicals for the management of plant-parasitic nematodes and soil-inhabiting fungi infesting chickpea. Turkish Journal of Agriculture and Forestry 36: 710-719.
Roman, D. L., D.I. Voiculescu, M. Filip, V. Ostafe and A. Isvoran. 2021. Effects of triazole fungicides on soil microbiota and on the activities of enzymes found in soil: A review. Agriculture 11(9): 893.
Rzedowski, C. and G. Rzedowski. 2001. Flora fanerogámica del Valle de México. Instituto de Ecología/ Comisión Nacional para el Conocimiento y Uso de la Biodiversidad. Michoacán, México.
Seepe, H.A., W. Nxumalo and S.O. Amoo. 2021. Natural products from medicinal plants against phytopathogenic Fusarium species: Current research endeavours, challenges and prospects. Molecules 26(21): 6539.
Singh A, Singh S, Singh S, Singh T, Singh V, Pandey V, Singh U. 2009. Fungal spore germination inhibition by alkaloids dehydrocorydalmine and oxyberberine. Journal of Plant Protection Research 49(3): 287-28.
Published
How to Cite
Issue
Section
Copyright (c) 2024 Jorge E. Ruiz-Nieto, Dulce M. Arias-Figueroa, Ana I. Mireles-Arriaga, Jesús Hernández-Ruíz
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Rights of the author/s are from the year of publication
This work is under the license:
Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
The opinions expressed by the authors not necesarily reflect the position of the publisher or UCLA. The total or partial reproduction of the texts published in this journal is authorized, as long as the complete source and the electronic address of this journal is cited. Authors have the right to use their articles for any purpose as long as it is done for non-profit purposes. Authors can publish the final version of their work on internet or any other medium, after it has been published in this journal.
Bioagro reserves the right to make textual modifications and technical adjustments to the figures of the manuscripts, in accordance with the style and specifications of the journal.
