Microscopic description of the foliar morphology of Neltuma chilensis (Molina) C.E. Hughes &G.P. Lewis
DOI:
https://doi.org/10.51372/bioagro381.4Keywords:
Foliar anatomy, histological description, histological techniques, morphologyAbstract
Neltuma chilensis, a native tree of Central Chile, is historically valued for its ecological and cultural importance, as well as for its resilience to drought. This study aims to characterize the microscopic foliar morphology of this species using histological techniques. Leaves were collected in the Region of Valparaíso, Chile, processed, and analyzed using various staining techniques, examining histological structures and staining affinity. A uniseriate epidermis with non-glandular, conical trichomes and stomata on the adaxial surface was observed; a dorsiventral mesophyll with palisade and spongy parenchyma; and a midrib containing lignified sclerenchyma, phloem, and xylem with tracheids. Dense, granular idioblasts containing mucilage were also identified, likely involved in water retention. These findings provide information on the anatomical structures associated with the adaptation of N. chilensis to extreme abiotic conditions, such as drought and climates with high fluctuations between maximum and minimum temperatures.
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Aguilar D.L., M.C. Acosta, M.C. Baranzelli, A.N. Sérsic, J. Delatorre-Herrera, A. Verga y A. Cosacov. 2020. Ecophylogeography of the disjunct South American xerophytic tree species Prosopis chilensis (Fabaceae). Biological Journal of the Linnean Society 129(4):793-809.
Aguilera, N. y L.M. Guedes. 2021. Manual de Técnicas Anatómicas e Histoquímicas para el Análisis de Muestras Vegetales. Universidad de Concepción. Chile.
Bancroft, J.D. y M. Gamble. 2008. Theory and practice of histological techniques (7th ed.). Churchill Livingstone/Elsevier.
Calderón Jiles, G.B. 2016. Prosopis tamarugo Phil. Un árbol endémico del desierto de atacama. Propuesta de niveles umbrales de profundidad freática para su conservación. Universidad de Chile. Memoria de Título Ingeniería en Recursos Naturales Renovables.
Carranza, C., G. Verzino, J. Di Rienzo, M. Ledesma, y J. Joseau, J. 2000. Componentes de la variación adaptativa en Prosopis Chilensis: el índice de brotación. Multequina 9(1): 55-64.
Contreras Díaz, R., M. González González, F. Aguayo Cruces, S. Gacitúa Arias y V. Porcile Saavedra. 2021. Análisis de diversidad y estructura genética de poblaciones de Prosopis chilensis entre las provincias de Chacabuco y San Felipe de Aconcagua, usando marcadores moleculares microsatélites. Bosque (Valdivia) 42(3): 371-382.
Coros, C. 2017. Los Arcaicos del Rutal: Tras la Explotación del Silex. Aguas Andinas / Museo Arqueológico de Los Andes.
Cui, X., Z. Chen, L. Shi, B. Wang, J. Guo, T. Zhang y W. Zhang. 2023. Comprehensive physiological, transcriptomic, and metabolomic analysis of the key metabolic pathways in millet seedling adaptation to drought stress. Physiologia Plantarum 175(6): e14122.
Emonet, A. y A. Hay. 2022. Development and diversity of lignin patterns. Plant Physiology 190(1): 31-43.
Fundación LEPE. 2024. Algarrobo. Huancu. Neltuma chilensis. https://n9.cl/b46fa (consulta de octubre 10, 2025).
Harrison, D., Q.J.A Guzmán y G.A. Sánchez-Azofeifa. 2021. Leaf Anatomical Traits of Lianas and Trees at the Canopy of Two Contrasting Lowland Tropical Forests in the Context of Leaf Economic Spectrum. Frontiers in Forests and Global Change 4:720813.
Hughes C.E., J.J. Ringelberg, G.P. Lewis y S.A. Catalano. 2022. Disintegration of the genus Prosopis L. (Leguminosae, Caesalpinioideae, mimosoid clade). In: Hughes CE, de Queiroz LP, Lewis GP (Eds) Advances in Legume Systematics 14. Classification of Caesalpinioideae Part 1: New generic delimitations. PhytoKeys 205: 147-189.
Johansen, D.A. 1940. Microtecnología vegetal. McGraw-Hill, Nueva York, US.
Karabourniotis, G., G. Liakopoulos, P. Bresta y D. Nikolopoulos. 2021. The optical properties of leaf structural elements and their contribution to photosynthetic performance and photoprotection. Plants 10(7):1455.
Kiernan, J.A. 2015. Kiernan’s histological and histochemical methods: Theory and practice. 5ta ed. Banbury: Scion Publishing.
Landl, M., D. Vetterlein, E. Kroener, J. Vanderborght, A. Schnepf, S. Schlüter y M. Phalempin. 2021. Modeling the Impact of Rhizosphere Bulk Density and Mucilage Gradients on Root Water Uptake. Frontiers in Agronomy 3: 622367.
Martínez, S. 2024. Plan de conservación circa situm de Neltuma chilensis (Molina) C.E. Hughes G.P. Lewis como una propuesta para cambiar su estado actual de amenaza. Santiago, Chile: Universidad de Chile-Facultad de Ciencias Forestales y de la Conservación de la Naturaleza.
Senilliani, M.G., C. Bruno y M. Brassiolo. 2020. Factores edáficos y su impacto en la altura dominante en plantaciones de Prosopis alba. Madera y Bosques 26(3): e2632054.
Soto Aguirre, D.A. y A.J. Gysling Caselli. 2009. Productos con oportunidades de desarrollo en Chile: mucílago de algarrobo chileno (Prosopis chilensis). Ciencia & Investigación Forestal 15(2): 255-276.
Villa-Uvidia, D.N., M.A. Osorio-Rivera y N.Y. Villacis-Venegas. 2020. Extracción, propiedades y beneficios de los mucílagos. Dominio de las Ciencias 6: 503-524.
Wall, S., S. Vialet-Chabrand, P. Davey, J. Van Rie, A. Galle, J. Cockram y T. Lawson. 2022. Stomata on the abaxial and adaxial leaf surfaces contribute differently to leaf gas exchange and photosynthesis in wheat. New Phytologist 235: 1743-175.
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Copyright (c) 2025 Cristian Fonseca-Salvo, Carlos Coros-Villca, Samuel Valdebenito, Guillermo Fernández-Bunster, Jonathan Aránguiz-Rodriguez
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