Response of potato varieties to different species of root-knot nematode (Meloidogyne spp.)
DOI:
https://doi.org/10.51372/bioagro382.12Keywords:
Inoculation, Meloidogyne, selection, susceptibility, resistanceAbstract
Potato is a fundamental crop for global food security; however, root-knot nematodes threaten its production. The aim of this study was to identify Meloidogyne species and assess the response of different potato varieties to this pathogen. Nematode populations were analyzed through biochemical characterization using esterase isoenzymes, identifying four species: M. incognita (Esterase I1, I2), M. arenaria (Est. A1, A2), M. hapla (Est. H1, H2), and M. ethiopica (Est. E3). Subsequently, thirteen potato cultivars were evaluated under greenhouse conditions, in pots, and inoculated with 5,000 eggs and J2 juveniles of nematodes. After 55 days, the number of galls, eggs, and the reproduction factor (RF) were measured. The Yungay cultivar exhibited the highest susceptibility, as evidenced by the high number of galls (25.68), eggs (74,633.3), and a high reproduction factor (RF) of 19.24. In contrast, the Ccompis, Venturana, and Ruki cultivars showed lower susceptibility, with the following results: galls (3.51, 3.56, and 1), eggs (1,783.3, 4,083.3, and 0), and RF (0.36, 0.472, and 0), respectively. Additionally, resistance was evaluated using three key indices: galls index (GI%), reproduction index (RI %), and RF. In this regard, the Yungay variety showed high susceptibility (IN=100%, IR=100%, RF=13.46), in contrast to resistant cultivars like Ruki (GI=5.5 %, RI=0 %, RF=0.00) and Andina (GI=15.3 %, RI=2.6 %, RF=0.35), which inhibited nematode penetration (GI%) and reproduction (RI %). This study provides key information on the resistance and susceptibility of potato cultivars to nematodes, which is useful for guiding management strategies and selecting appropriate varieties in areas with a high presence of root-knot nematodes.
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Alarcón, R.S.M. 2017. Identificación de la especie de nematodo (Globodera spp.) mediante PCR y resistencia de 49 accesiones de papa nativa (Solanum spp.) a 3,379 m.s.n.m. Campanayocc-Ayacucho. UNSCH. https://shre.ink/e3uQ
Ali, M.A., F. Azeem, H. Li and H. Bohlmann. 2021. Smart parasitic nematodes use multifaceted strategies to parasitize plants. Frontiers in Plant Science 8, 21 p
Bridge, J., M. Luc and R.A. Plowright. 2005. Nematode parasites of tropical and subtropical roots and tuber crops. In: M. Luc, R. A. Sikora, & J. Bridge (eds.), Plant parasitic nematodes in subtropical and tropical agriculture. CABI Pub. pp. 221-258.
Carneiro, R.M.D.G and M.R.A. Almeida. 2001. Técnica de eletroforese usada no estudo de enzimas dos nematóides de galhas para identificação de espécies. Nematologia Brasileira 25(1): 35-44.
Carreño, H., O. Ponce, R. Casanova, G. de la Cruz and E. Neyra. 2020. Perfil del transcriptoma de raíz de papa resistente y susceptible a Globodera pallida. Revista Peruana de Biología 27(1): 1-12.
Chang, D.C., H. B. Sohn, J.H. Cho, J.S. Im, Y.I. Jin, G.R. Do et al. 2014. Freezing and Frost Damage of Potato Plants: a Case Study on Growth Recovery, Yield Response, and Quality Changes. Potato Res. 57: 99-110.
Coolen, W.A. and C.J. D'herde. 1972. A method for the quantitative extraction of nematodes from plant tissue Ghent: State Agricultural Research Center 77 p.
Dandurand, S.R., L.R. Zasada, I.A. Franco and J.C. Kuhl. 2022. An assessment of potato cyst nematode (Globodera spp.) research from the Andean region of South America. Part 1: Occurrence and impact. Nematropica 51(2): 1-15.
Devindrappa, M., B. Singh, R. Jagadeeswaran and T. Basavaraja. 2025. Effect of Root-knot Nematode (Meloidogyne javanica) Infection on Mungbean Genotypes: Identification and Characterization of Host Plant Resistance. Legume Research: An International Journal 48(2): 123-130.
Devran, Z., N. Mutlu and I.H. Elekçioğlu. 2019. Identification and genetic diversity of Meloidogyne chitwoodi in potato production areas of Turkey. Plant Disease 93(3): 316.
Elling, A.A. 2013. Major emerging problems with minor Meloidogyne species. Phytopathology 103(11): 1092-1102.
Flores, C.Y., R.Y.B. Portocarrero and I.L. Medina. 2017. Prospección de Nematodos fitoparasitos en cultivo de papa (Solanum tuberosum L.) de la Region de Puno. Journal of Hogh Andean Research 19(1): 11-20.
Goverse, A. and S.Geert. 2014. The activation and supression of plant innate immunity by parasitic nematodes. Annual Review of Phytopathology 52: 243-265.
Hussain, M., T. Mukhtar and M. Kayani. 2016. Reproduction of Meloidogyne incognita on resistant and susceptible okra cultivars. Pakistan Journal of Agricultural Sciences 53(2): 371-375.
Jones, J.T., A. Haegeman, E.G.J. Danchin, H.S. Gaur, J. Helder, M.G.K Jones et al. 2013. Top 10 plant‐parasitic nematodes in molecular plant pathology. Molecular Plant Pathology 14(9): 946-961.
Karssen, G., W.M.L. Wesemael, and M. Moens. 2013. Root-knot nematodes. In: R. N. Perry and M. Moens (Eds.). Plant Nematology. CABI Pub. pp. 73-108.
Khan, A., S.A. Ansari, M. Haris, T. Hussain and A.A. Khan. 2023. Meloidogyne species: Threat to vegetable produce. En: F. Ahmad & G.N. Blázquez (Eds.), Root-Galling Disease of Vegetable Plants. pp. 17-38. Springer.
Kumar, V. and B. Singh. 2021. Resistance of different cultivars to Meloidogyne spp.: A review on nematode damage and plant defense mechanisms. Journal of Nematology Research 45(3): 120-134.
Kim, E., Y. Seo, Y. Yong, S.K. Yong and Y.H. Kim. 2017. Effects of soil textures on infectivity of root-knot nematodes on carrot. Plant Pathology 1(33): 66-74.
Lima, F.S.O. Mattos, V.S. Silva, E.S. Carvalho and M.A.S. 2018. Nematodes affecting potato. In Book Potato–From incas to all over world. 107-124.
Maleita, C., J.M.S. Cardoso, L. Rusinque. I. Esteves and I. Abrantes. 2021. Species-specific molecular detection of the root knot nematode Meloidogyne luci. Biology (Basel) 10(8):775.
Medina, I.L, C.B. Gomes, V.R. Correa, V.S. Mattos, P. Castagnone-Sereno and R.M.D.G. Carneiro. 2017. Genetic diversity of Meloidogyne spp. Parasitising potato in Brazil and aggressiveness of M. Javanica population on susceptible cultivars. Nematology 19(1): 69-80.
Oliveira, C. and J. Rosa. 2021. Nematoides parasitos da batata. In book: Batata: Desafios Fitossanitários e Manejo Sustentável 231-248 https://n9.cl/9owaq
Oostenbrink, M. 1966. Major characteristics of the relation between nematodes and plants (Mededelingen / Landbouwhoge, Wageningen No. 66-4). Veenman. 48 p. https://shre.ink/e35u
Potter, C., E.M. Sarapura, Z.P. Vander, M. Salari and T. Zink. 2024. Best management practice adoption amongst potato producers in Ontario: A study of drivers and barriers. Frontiers in Sustainable Food Systems 8: 1358515.
Ramatsitsi, N. and K. Ramachela. 2023. Histological characterization of wild cucumber resistance to Meloidogyne species. J. Plant Dis. Prot. 130: 883-889.
Ramos. Q.J. 2020. Resistencia de cultivares nativas de papa (Solanum spp.) al nematodo Meloidogyne spp. en condiciones controladas. Tesis de maestría, Universidad Nacional Agraria La Molina. Perú. https://shre.ink/e3Aj
Salazar, A. W. and T.J. Hernández. 2013. Efecto de poblaciones de Meloidogyne sp. en el desarrollo y rendimiento del tomate. Agronomía Mesoamericana 24: 419
Sasser, J.N. 1980. Root-Knot Nematodes: A Global Problem in Agriculture. Plant Disease 64: 8-12.
Schaffer, J.T. Gomes, C.B. Pereira, A.S. Cruz, F.C. Barros, D.R. 2017. Resistance of potato genotypes to Meloidogyne javanica. Revista Caatinga 30(2): 536-540.
Sikora, R.A., D. Coyne, J. Hallmann and P. Timper. 2018. Plant parasitic nematodes in subtropical and tropical agriculture. CABI.
Seid, A., C. Fininsa, T.M. Mekete, W. Decraemer and W.M.L. Wesemael. 2019. Damage potential of Meloidogyne incognita populations of selected tomato genotypes in Ethiopia. Nematropica 49: 124-133.
Spooner, D.M., K. McLean, G. Ramsay, R. Waugh and G.J. Bryan. 2005. The history of the potato and its cultivation in the Andes. En J. Janick (ed.), Horticultural Reviews John Wiley & Sons. N.Y. Vol 31, 72 p.
Spychalla, P., and W.S. De Jong. 2024. Breeding for potato cyst nematode resistance in Solanum tuberosum. Crop Science 64: 1167–1182.
Teklu, M.G., C.H. Schomaker, H.B.T.H. Thomas and P.G.M Leendert,. 2023. Quality and Infestation Levels of Potato Genotypes, Resistant to the Root-Knot Nematode, Meloidogyne chitwoodi. Potato Res. 66: 105-135.
Trudgill, D.L. 1991. Resistance to and tolerance of plant parasitic nematodes in plants. Annual Review of Phytopathology 29: 167-192.
Wegulo, S.N., J.A. Breathnach and P.S. Baenziger. 2009. Effect of growth stage on the relationship between tan spot and spot blotch severity and yield in winter wheat. Crop Proteccion 28(8): 696-702.
Williamson, V.M. and R.S. Hussey. 1996. Nematode pathogenesis and resistance in plants. The Plant Cell 8(10): 1735-1745.
Williamson, V.M. and A. Kumar. 2006. Nematode resistance in plants: The battle underground. Trends in Genetics 227: 396-403.
Zapana, C.A.J. 2024. Respuesta de variedades de papa nativa al nematodo nódulo de la raíz (Meloidogyne spp.) en condiciones de invernadero. Tesis de Grado, Universidad Nacional del Altiplano. Perú. 110 p. https://repositorio.unap.edu.pe/handle/20.500.14082/23762
Zhang, L. and C. Gleason. 2020. Enhancing potato resistance against root-knot nematodes using a plant-defence elicitor delivered by bacteria. Nature Plants 6: 625-629.
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