Hydrogen peroxide and the development of mango seedlings cv. Criollo de Chulucanas

Authors

  • Luis Nole Alama Departamento de Morfofisiología Vegetal, Universidad Nacional de Piura, Campus Universitario s/n, Urbanización Miraflores Piura, Perú. https://orcid.org/0009-0002-8486-2734
  • Rodrigo López Criollo Departamento de Morfofisiología Vegetal, Universidad Nacional de Piura, Campus Universitario s/n, Urbanización Miraflores Piura, Perú. https://orcid.org/0009-0009-6782-6492
  • Junior Juarez Portuguez Departamento de Morfofisiología Vegetal, Universidad Nacional de Piura, Campus Universitario s/n, Urbanización Miraflores Piura, Perú. https://orcid.org/0009-0008-8367-9296
  • Roger Chanduví-García Departamento de Morfofisiología Vegetal, Universidad Nacional de Piura, Campus Universitario s/n, Urbanización Miraflores Piura, Perú. https://orcid.org/0000-0002-6061-3007
  • Carlos San Martin-Zapata Departamento de Morfofisiología Vegetal, Universidad Nacional de Piura, Campus Universitario s/n, Urbanización Miraflores Piura, Perú. http://orcid.org/0000-0002-3861-8119
  • Arturo Morales-Pizarro Departamento de Morfofisiología Vegetal, Universidad Nacional de Piura, Campus Universitario s/n, Urbanización Miraflores Piura, Perú. http://orcid.org/0000-0003-3966-6689

DOI:

https://doi.org/10.51372/bioagro382.11

Keywords:

Emergence, Mangifera indica, seedling vigor

Abstract

Mango is one of the most important agricultural export products in northern Peru. Therefore, it is necessary to think about methods for producing high-quality seedlings to ensure good crop development. The objective of this study was to investigate the effect of hydrogen peroxide on the emergence and initial growth of mango seedlings. Four concentrations (C) of the product (0, 10, 30, and 50 % H₂O₂) and two imbibition times (T) (6 and 12 h) were evaluated in a factorial arrangement for a total of eight treatments in mango ‘Criollo de Chulucanas’ (apomixis: polyembryony). For the evaluation, the most vigorous shoots and main root were selected. The percentage of emergence (PE), main root length (RL), plant height (PH), main root diameter (RD), stem diameter (SD), number of leaves (NL), shoot and root fresh weight (SFW and RFW), and total soluble solids of root (TSSr) were determined. The results showed a positive effect of treatment C2*T1 on PE, RD, RL, SFW and RFW, and of C4*T1 and C4*T2 on TSSr. Treatment C3*T2 increased SD. These same relationships were observed in the principal component analysis (PCA) and cluster analysis. Correlation analysis showed that PE was positively correlated with RD and RFW, as was RD with SFW, and RL with RFW. The variable TSSr showed a negative correlation with SFW. It is concluded that seed treatment with hydrogen peroxide is a safe alternative to stimulate seedling emergence and development in mango.

Downloads

Download data is not yet available.

References

Abdelshafy, A., M.H. Neetoo y F. Al-Asmari. 2024. Antimicrobial activity of hydrogen peroxide for application in food safety and COVID-19 mitigation: An updated review. Journal of Food Protection 87(7): 100306.

Abdul Mazeed, N. 2025. Role of Hydrogen Peroxide on Horticultural Crops in Hydrogen Peroxide. CRC Press 198-207.

Bahin, E., C. Bailly, B. Sotta, I. Kranner, F. Corbineau y J. Leymarie. 2011. Crosstalk between reactive oxygen species and hormonal signalling pathways regulates grain dormancy in barley. Plant, Cell & Environment 34(6): 980-993.

Bewley, J.D., K.J. Bradford, H.M.W. Hilhorst y H. Nonogaki. 2013. Seeds: Physiology of Development, Germination and Dormancy. New York, NY: Springer.

Cardoza, A., D. Arévalo-Valladolid, J. Javier-Alva, R. Peña-Castillo, R. Chanduví-García, M. Quiroz-Calderón y A. Morales-Pizarro. 2024. Alternative Organic Substrates in the Germination and Initial Growth of Corn (Zea mays L,) Seedlings Under Nursery Conditions. Terra Latinoamericana 42: 1-11.

Chu, C., R.C. Poore, M.D. Bolton y K.K. Fugate. 2022. Mechanism of sugarbeet seed germination enhanced by hydrogen peroxide. Frontiers in Plant Science 13: 888519.

CIEN (Centro de Investigación de Economía y Negocios Globales). 2025. Nota de inteligencia comercial. Mango: evolución del mercado Internacional y Nacional. https://n9.cl/yqypuu

Deng, Y., C. Wang, N. Wang, L. Wei, W. Li y W. Liao. 2019. Roles of small-molecule compounds in plant adventitious root development. Biomolecules 9(9): 420.

Diaz, P., G. Barba-Espín y J.A. Hernández. 2013. Elucidating hormonal/ROS networks during seed germination: insights and perspectives. Plant Cell Reports 32: 1491-1502.

Dolatabadian, A., y S.A.M.M. Sanavy. 2008. Effect of the ascorbic acid, pyridoxine and hydrogen peroxide treatments on germination, catalase activity, protein and malondialdehyde content of three oil seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 36(2): 61-66.

Domingues, F.J., D.C.D.S. Carneiro, F.F. Putti, J.D. Rodrigues, M.A. Tecchio, S. Leonel y M.D.S. Silva. 2024. Physiological indexes in seed germination and seedling growth of Rangpur lime (Citrus limonia L. Osbeck) under plant growth regulators. Agronomy 14(9): 2066.

FAO (Food and Agriculture Organization). 2025. Crops and livestock products. https://n9.cl/ad5u9

GBIF (Global Biodiversity Information Facility). 2023. Mangifera indica L. https://www,gbif,org/es/species/3190638

Hameed, A., S. Farooq, S. Iqbal y R. Arshad. 2004. Influence of exogenous application of hydrogen peroxide on root and seedling growth on wheat (Triticum aestivum L.). International Journal of Agriculture and Biology 6(2). https://n9.cl/wg1g5

Jamaludin, R., N. Mat, K. Suryati Mohd, N. Afiza Badaluddin, K. Mahmud, M. Hailmi Sajili y M.M. Khandaker. 2020. Influence of exogenous hydrogen peroxide on plant physiology, leaf anatomy and rubisco gene expression of the Ficus deltoidea Jack va.r Deltoidea. Agronomy 10(4): 497.

Li, S., L. Xue, S. Xu, H. Feng y L. An. 2007. Hydrogen peroxide involvement in formation and development of adventitious roots in cucumber. Plant Growth Regulation 52: 173-180.

Merino-Valdés, M., P. Andrés-Meza, O.R. Leyva-Ovalle, H. López-Sánchez, J. Murguía-González, R. Núñez-Pastrana y J. Luis. 2018. Influencia de tratamientos pregerminativos en semillas de chile manzano (Capsicum pubescens Ruiz & Pav.). Acta Agronómica 67(4): 531-537.

MIDAGRI (Ministerio Desarrollo Agrario y Riego). 2023. Ficha de requerimientos agroecológicos de mango. https://n9.cl/bcds1o

Mostafa, L. 2021. Effect of Hydrogen Peroxide on the Growth, Fruit Set, Yield and Quality of Ewais Mango Trees. Egyptian Journal of Horticulture 48(1): 71-81.

Morales, A., D. Rondoy-Castro, P. Escobedo-Huancas, E. Durand-Montejo, A. Veramatus-Mendoza, J. Juárez-Vilchez y R. Peña Castillo. 2025. Dose and imbibition times of hydrogen peroxide in germination and initial growth of cotton (Gossypium barbadense L.). Tropical and Subtropical Agroecosystems 28(1).

Morocho, H., R. Peña-Castillo, R. Chanduvi-García, S. Vilchez-Navarro, M. Quiroz-Calderón, M. Calero-Merino et al. 2024. Influence of organic fertilization on the fruit morphology and production of mango (Mangifera indica L.) var. Kent,.Tropical and Subtropical Agroecosystems 27(3).

RStudio Team. 2025. RStudio: Integrated Development Environment for R (Versión 2025.09.2+418). https://n9.cl/bl3ri

Ruesta-López, M., L. Zurita-Chinguel, M. Lizano-Pintado, M. Delgado-Vite, D. Zapata-Durand, R. Peña-Castillo et al. 2024. Biostimulant and imbibition times on passion fruit (Passiflora edulis s. passifloraceae) seed germination. Tropical and Subtropical Agroecosystems 27(2).

Song, K.E., H.R. Hwang, E.S.H. Hong, P. Konvalina, W.J. Jun, J.W. Jung y S. Shim. 2023. Hydrogen peroxide ameliorates the adversities of drought stress during germination and seedling growth in sorghum (Sorghum bicolor L.). Agronomy 13(2): 330.

Veloso, L.L.D.S.A., J.D. Capitulino, C.A.V. De Azevedo, A.A.R. Da Silva y H.R. Gheyi. 2021. Application methods of hydrogen peroxide in soursop seedlings irrigated with saline water. Comunicata Scientiae 12: 1-8.

Wojtyla, Ł., K. Lechowska, S. Kubala y M. Garnczarska. 2016. Different modes of hydrogen peroxide action during seed germination. Frontiers in Plant Science 7: 66.

Xiong, J., Y. Yang, G. Fu y L. Tao. 2015. Novel roles of hydrogen peroxide (H2O2) in regulating pectin synthesis and demethylesterification in the cell wall of rice (Oryza sativa) root tips. New Phytologist 206(1): 118-126.

Zeinalabedini, M., K. Majourhat, M. Khayam-Nekoui, J.A. Hernández y P. Martínez-Gómez. 2009. Breaking seed dormancy in long-term stored seeds from Iranian wild almond species. Seed Science and Technology 37(2): 267-275.

Published

2026-05-01

How to Cite

Nole Alama, L., López Criollo, R., Juarez Portuguez, J., Chanduví-García, R., San Martin-Zapata, C., & Morales-Pizarro, A. (2026). Hydrogen peroxide and the development of mango seedlings cv. Criollo de Chulucanas. Bioagro, 38(2), 275-284. https://doi.org/10.51372/bioagro382.11

Issue

Section

Artículos