Respuesta de enzimas oxidantes y antioxidantes del sorgo dulce var. Redbine bajo estrés salino de NaCl
Palabras clave:
Malondialdehído, proline, estrés salinoResumen
El sorgo posee compuestos bioactivos benéficos para la salud humana. Se investigó la tolerancia al NaCl de la variedad Redbine a través de antioxidantes enzimáticos y no enzimáticos. Las plantas recibieron solución de Hoagland con 0-200 mM de la sal por 10 días y se evaluó la actividad enzimática y contenido de clorofila y carotenos. La catalasa (CAT), ascorbato peroxidasa (APX), glutatión reductasa (GR) y la prolina indicaron un aumento en dosis de 50-150 mM que indicó la respuesta protectora de la planta. La actividad de la superóxido dismutasa (SOD) y la cantidad de peroxidación lipídica de membrana (malondialdehído, MDA) revelaron un aumento en todas las concentraciones tanto en las raíces como en las hojas. APX, GR, glutatión S-transferasa (GST), MDA y prolina en hojas y raíces también mostraron tendencia creciente. La mayor actividad de SOD ocurrió a 200 mM de la sal y la síntesis de clorofilas y caroteno se produjo en todas las concentraciones, indicando que la planta mostró respuesta efectiva contra el estrés salino. Aunque las actividades enzimáticas antioxidantes del sorgo contribuyen a su respuesta al estrés salino, esto no parece ser adecuada a concentraciones más altas.
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20. Madhava R.K. and T.V. Sresty. 2000. Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Science 157: 113-128.
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22. Misra, N and A.K. Gupta. 2006. Effect of salinity and different nitrogen sources on the activity of antioxidant enzymes and indole alkaloid content in Catharanthus roseus seedlings. Journal of Plant Physiology 163: 11-18.
23. Mittal, S., N. Kumari and V. Sharma. 2012. Differential response of salt stress on Brassica juncea: Photosynthetic performance, pigment, proline, D1 and antioxidant enzymes. Plant Physiology and Biochemistry 54: 17-26.
24. Munné-Bosch, S and L. Alegre. 2004. Die and let live: Leaf senescence contributes to plant survival under drought stress. Functional Plant Biology 31: 203-216.
25. Mutegi, E., F. Sagnard., M. Muraya., B. Kanyenji., B. Rono., C. Mwongera et al. 2010. Ecogeographical distribution of wild, weedy and cultivated Sorghum bicolor (L.) Moench in Kenya: implications for conservation and crop-to-wild gene flow. Genetenetic Resources and Crop Evolution 57: 243-253.
26. Nounjan, N., P.T. Nghia and P. Theerakulpisut. 2012. Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes. Journal of Plant Physiology 169: 596-604.
27. Ramanjulu, S and D. Bartels. 2002. Drought- and desiccation-induced modulation of gene expression in plants. Plant, Cell and Environment 25: 141-151.
28. Scandalios, J.G. 1993. Oxygen Stress and Superoxide Dismutases. Plant Physiology 101: 7-12.
29. Shigeoka, S., T. Ishikawa., M. Tamoi., Y. Miyagawa., T. Takeda., Y. Yabuta and K. Yoshimura. 2002. Regulation and function of ascorbate peroxidase isoenzymes. Journal of Experimental Botany 53: 1305-1319.
30. Sun, W., X. Xu., H. Zhu., A. Liu., L. Liu., J. Li., and X. Hua. 2010. Comparative transcriptomic profiling of a salt-tolerant wild tomato species and a salt-sensitive tomato cultivar. Plant and Cell Physiology 51: 997-1006.
31. Temizgul, R., M. Kaplan., R. Kara and S. Yilmaz. 2016. Effects of salt concentrations on antioxidant enzyme activity of grain sorghum. Current Trends in Natural Sciences 5: 171-178.
32. Tewari, R.K., P. Kumar., P.N. Sharma and S.S. Bisht. 2002. Modulation of oxidative stress responsive enzymes by excess cobalt. Plant Science 162: 381-388.
33. Yadav, S.K. 2010. Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. South African Journal of Botany 76: 167-179.
34. Yamane, K., S. Mitsuya., M. Taniguchi and H. Miyake. 2010. Transcription profiles of genes encoding catalase and ascorbate peroxidase in the rice leaf tissues under salinity. Plant Production Science 13: 164-168.
35. Yan, K., H. Xu., W. Cao and X. Chen. 2015. Salt priming improved salt tolerance in sweet sorghum by enhancing osmotic resistance and reducing root Na+ uptake. Acta Physiologiae Plantarum 37: 203. 10 p.
36. Yildiz, M., H. Terzi., E. Bilgisi., A. Makalesi., B. Üretim and S. Yazar. 2013. Effect of NaCl stress on chlorophyll biosynthesis, proline, lipid peroxidation and antioxidative enzymes in leaves of salt-tolerant and salt-sensitive barley cultivars. Journal of Agricultural Sciences 19: 79-88.
37. Yilmaz, S.H., M. Kaplan., R. Temizgul and S. Yilmaz. 2017. Antioxidant enzyme response of sorghum plant upon exposure to aluminum, chromium and lead heavy metals. Turkish Journal of Biochemistry 42: 503-512.
38. Yousuf, P.Y., K.U.R. Hakeem., R. Chandna and P. Ahmad. 2012. Role of glutathione reductase in plant abiotic stress. In: Abiotic Stress Responses in Plants. Springer. New York. pp. 149-158.
39. Zhang, F.Q., Y.S. Wang., Z.P. Lou and J.D. Dong. 2007. Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere 67: 44-50.
2. AbdElgawad, H., G. Zinta., M.M. Hegab., R. Pandey., H. Asard and W. Abuelsoud. 2016. High salinity induces different oxidative stress and antioxidant responses in maize seedlings organs. Frontiers in Plant Science 7: 276.
3. Akbulut, M. and S. Çakır. 2010. The effects of Se phytotoxicity on the antioxidant systems of leaf tissues in barley (Hordeum vulgare L.) seedlings. Plant Physiology and Biochemistry 48: 160-166.
4. Arshi, A., A. Ahmad., I.M. Aref and M. Iqbal. 2012. Comparative studies on antioxidant enzyme action and ion accumulation in soybean cultivars under salinity stress. Journal of Environmental Biology 33: 9-20.
5. Asada, K. 1984. Chloroplasts: Formation of active oxygen and its scavenging. Methods in Enzymology 105: 422-429.
6. Balaji, M., A. Ediga., S. Hemalatha and B. Meriga. 2013. Effect of salinity stress on antioxidant defense system of two finger millet cultivars (Eleusine coracana (L.) Gaertn) differing in their sensitivity. Advances in Biological Research 7: 180-187.
7. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
8. Csiszár J., Z. Váry., A. Gallé., K. Bela., S. Brunner and I. Tari. 2014. Glutathione transferase supergene family in tomato: Salt stress-regulated expression of representative genes from distinct GST classes in plants primed with salicylic acid. Plant Physiology and Biochemistry 78:15-26.
9. De Morais Cardoso, L., S.S. Pinheiro., H.S.D. Martino and H.M. Pinheiro-Sant’Ana. 2017. Sorghum (Sorghum bicolor L.): Nutrients, bioactive compounds, and potential impact on human health. Critical Reviews in Food Science and Nutrition 57: 372-390.
10. Demiral, T. and İ. Türkan. 2005. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany 53: 247-257.
11. Eyidogan, F. and M.T. Öz. 2007. Effect of salinity on antioxidant responses of chickpea seedlings. Acta Physiologiae Plantarum 29: 485-493.
12. García, M., G. García, J. Hernández and A. Pieters. 2019. Oxidative damage and antioxidant behaviour of ascorbate and glutathione in two onion genotypes differing in salt sensitivity. Bioagro 31(2): 81-90.
13. Gharsallah, C., H. Fakhfakh., D. Grubb and F. Gorsane. 2016. Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars. AoB Plants 8, plw055. 21 p.
14. Giannopolitis, C.N and S.K. Ries. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant Physiology 59: 309-14.
15. Hare, P.D and W.A. Cress. 1997. Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regulion 21: 79-102.
16. Hernandez, J.A and M.S. Almansa. 2002. Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves. Physiologia Plantarum 115: 251-257.
17. Hiscox A.N. and D.G. Israelstam. 1979. Method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1332-1334.
18. Huang, R. 2018. Research progress on plant tolerance to soil salinity and alkalinity in sorghum. Journal of Integrative Agriculture 17: 739-746.
19. Karuppanapandian, T., J.C. Moon., C. Kim., K. Manoharan and W. Kim. 2011. Reactive oxygen species in plants: Their generation, signal transduction, and scavenging mechanisms. Australian Journal of Crop Science 5: 709-725.
20. Madhava R.K. and T.V. Sresty. 2000. Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Science 157: 113-128.
21. Menezes-Benavente, L., F.K. Teixeira, C.L. Alvim Kamei and M. Margis-Pinheiro. 2004. Salt stress induces altered expression of genes encoding antioxidant enzymes in seedlings of a Brazilian indica rice (Oryza sativa L.). Plant Science 166: 323-331.
22. Misra, N and A.K. Gupta. 2006. Effect of salinity and different nitrogen sources on the activity of antioxidant enzymes and indole alkaloid content in Catharanthus roseus seedlings. Journal of Plant Physiology 163: 11-18.
23. Mittal, S., N. Kumari and V. Sharma. 2012. Differential response of salt stress on Brassica juncea: Photosynthetic performance, pigment, proline, D1 and antioxidant enzymes. Plant Physiology and Biochemistry 54: 17-26.
24. Munné-Bosch, S and L. Alegre. 2004. Die and let live: Leaf senescence contributes to plant survival under drought stress. Functional Plant Biology 31: 203-216.
25. Mutegi, E., F. Sagnard., M. Muraya., B. Kanyenji., B. Rono., C. Mwongera et al. 2010. Ecogeographical distribution of wild, weedy and cultivated Sorghum bicolor (L.) Moench in Kenya: implications for conservation and crop-to-wild gene flow. Genetenetic Resources and Crop Evolution 57: 243-253.
26. Nounjan, N., P.T. Nghia and P. Theerakulpisut. 2012. Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes. Journal of Plant Physiology 169: 596-604.
27. Ramanjulu, S and D. Bartels. 2002. Drought- and desiccation-induced modulation of gene expression in plants. Plant, Cell and Environment 25: 141-151.
28. Scandalios, J.G. 1993. Oxygen Stress and Superoxide Dismutases. Plant Physiology 101: 7-12.
29. Shigeoka, S., T. Ishikawa., M. Tamoi., Y. Miyagawa., T. Takeda., Y. Yabuta and K. Yoshimura. 2002. Regulation and function of ascorbate peroxidase isoenzymes. Journal of Experimental Botany 53: 1305-1319.
30. Sun, W., X. Xu., H. Zhu., A. Liu., L. Liu., J. Li., and X. Hua. 2010. Comparative transcriptomic profiling of a salt-tolerant wild tomato species and a salt-sensitive tomato cultivar. Plant and Cell Physiology 51: 997-1006.
31. Temizgul, R., M. Kaplan., R. Kara and S. Yilmaz. 2016. Effects of salt concentrations on antioxidant enzyme activity of grain sorghum. Current Trends in Natural Sciences 5: 171-178.
32. Tewari, R.K., P. Kumar., P.N. Sharma and S.S. Bisht. 2002. Modulation of oxidative stress responsive enzymes by excess cobalt. Plant Science 162: 381-388.
33. Yadav, S.K. 2010. Heavy metals toxicity in plants: An overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. South African Journal of Botany 76: 167-179.
34. Yamane, K., S. Mitsuya., M. Taniguchi and H. Miyake. 2010. Transcription profiles of genes encoding catalase and ascorbate peroxidase in the rice leaf tissues under salinity. Plant Production Science 13: 164-168.
35. Yan, K., H. Xu., W. Cao and X. Chen. 2015. Salt priming improved salt tolerance in sweet sorghum by enhancing osmotic resistance and reducing root Na+ uptake. Acta Physiologiae Plantarum 37: 203. 10 p.
36. Yildiz, M., H. Terzi., E. Bilgisi., A. Makalesi., B. Üretim and S. Yazar. 2013. Effect of NaCl stress on chlorophyll biosynthesis, proline, lipid peroxidation and antioxidative enzymes in leaves of salt-tolerant and salt-sensitive barley cultivars. Journal of Agricultural Sciences 19: 79-88.
37. Yilmaz, S.H., M. Kaplan., R. Temizgul and S. Yilmaz. 2017. Antioxidant enzyme response of sorghum plant upon exposure to aluminum, chromium and lead heavy metals. Turkish Journal of Biochemistry 42: 503-512.
38. Yousuf, P.Y., K.U.R. Hakeem., R. Chandna and P. Ahmad. 2012. Role of glutathione reductase in plant abiotic stress. In: Abiotic Stress Responses in Plants. Springer. New York. pp. 149-158.
39. Zhang, F.Q., Y.S. Wang., Z.P. Lou and J.D. Dong. 2007. Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere 67: 44-50.
Publicado
2020-05-15
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Semih, Ridvan, Cengiz, & Mahmut. (2020). Respuesta de enzimas oxidantes y antioxidantes del sorgo dulce var. Redbine bajo estrés salino de NaCl. Bioagro, 32(1), 31-38. Recuperado a partir de https://revistas.uclave.org/index.php/bioagro/article/view/2684
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