Pseudomonas putida KT2440 induce tolerancia a la sequía durante la maduración del tomate
DOI:
https://doi.org/10.51372/bioagro342.4Palabras clave:
Bacterias promotoras del crecimiento, enzimas antioxidantes, estrés por sequía, Solanum lycopersicumResumen
El estudio investigó los efectos de la cepa KT2440 de Pseudomonas putida sobre la tolerancia a la sequía de plantas de tomate durante la maduración de la fruta. Las plantas en etapa de fruta verde madura no se regaron durante 20 días para promover el estrés por sequía. Se determinaron los contenidos de pigmentos fotosintéticos. Las plantas que recibieron la bacteria (PCB) tuvieron mayor cantidad de clorofila y carotenoides que las plantas sin la bacteria (PSB) en condiciones de estrés. En comparación con las PSB, la sequía indujo menor conductancia estomática, peroxidación de lípidos y peróxido de hidrógeno, y mayor actividad de ascorbato peroxidasa (APX), catalasa (CAT) y peroxidasa (POD) en las PCB. El número y peso de los frutos en las PCB y PSB se redujo por el estrés por sequía, pero la reducción fue menor en las PCB. Estos resultados indican que el tratamiento con bacterias confirió tolerancia al estrés por sequía en plantas de tomate al reducir el grado de peroxidación de los lípidos polares (PLs), aumentar el contenido de pigmentos fotosintéticos y las actividades de las enzimas antioxidantes en las hojas. Se concluye que P. putida KT2440 facilitó un alto rendimiento de frutos bajo estrés por sequía como un inductor biótico de tolerancia a este tipo de estrés.
Descargas
Citas
Abdelaal, K., M. AlKahtani, K. Attia, Y. Hafez, L. Királyand and A. Künstler. 2021. The role of plant growth-promoting bacteria in alleviating the adverse effects of drought on plants. Biology 10(6): 520.
Acharya, B.R. and S.M. Assmann. 2009. Hormone interactions in stomatal function. Plant Molecular Biology 69: 451-462.
Adesemoye, A.O. and D. Egamberdieva. 2013. Beneficial effects of plant growth-promoting rhizobacteria on improved crop production: prospects for developing economies. In: D.K. Maheshwari, M. Saraf and A. Aeron (eds.). Bacteria in Agrobiology: Crop Productivity. Springer, Berlin. pp. 45-63.
Aebi, H. 1983. Catalase. In: H. Bergmeyer (ed.). Methods of Enzymatic Analysis. Verlag Chemie, Germany. pp. 223-227.
Amari, T. and C. Abdelly. 2021. Biochemical responses of Digitaria commutata and Cenchrus ciliaris to water stress: antioxidative reactions, proline and soluble sugars accumulation. Bioagro 33(3): 171-180.
Arkhipova, T.N., E. Prinsen, S.U. Veselov, E.V. Martineko, A.I. Melentiev and GR. Kudoyarova. 2007. Cytokinin producing bacteria enhances plant growth in drying soil. Plant and Soil 292: 305-315.
Arnon, D. 1949. Copper enzymes in isolated chloroplasts: polyphenol oxidases in Beta vulgaris. Plant Physiology 24: 1-15.
Arshad, M., B. Shaharoona and T. Mahmood. 2008. Inoculation with Pseudomonas spp. containing acc-deaminase partially eliminates the effects of drought stress on growth, yield, and ripening of pea (Pisum sativum L.). Pedosphere 18: 611-620.
Arzanesh, M., H. Alikhani, K. Khavazı, H. Rahimian and M. Miransari. 2011. Wheat (Triticum aestivum L.) growth enhancement by Azospirillum sp. under drought stress. World Journal of Microbiology and Biotechnology 27: 197-205.
Bhattacharyya, C., S. Banerjee, U. Acharya, A. Mitra, I. Mallick, A. Haldar et al. 2020. Evaluation of plant growth promotion properties and induction of antioxidative defense mechanism by tea rhizobacteria of Darjeeling, India. Scientific Reports 10: 15536.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72: 248-254.
Campos, H., C. Trejo, C.B. Pena-Valdivia, R. Garcia-Nava, F.V. Conde-Martinez, and M.R. Cruz-Ortega. 2014. Stomatal and non-stomatal limitations of bell pepper (Capsicum annuum L.) plants under water stress and re-watering: Delayed restoration of photosynthesis during recovery. Environmental and Experimental Botany 98: 56-64.
Chakraborty, U. and B. Pradhan. 2012. Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation. Brazizilian Journal of Plant Physiology 24: 117-130.
Chakraborty, U., B.N. Chakraborty, A.P. Chakraborty and P.L. Dey. 2013. Water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria. World Journal of Microbiology and Biotechnology 29: 789-803.
Cohen, A., R. Bottini and P. Piccoli. 2008. Azospirillum brasilense Sp produces ABA in chemically-defined culture medium and increases ABA content in Arabidopsis plants. Plant Growth Regulation 54: 97-103.
De Jong, M., C. Mariani and W.H. Vriezen. 2009. The role of auxin and gibberellin in tomato fruit set. Journal of Experimental Botany 60: 1523-1532.
Delshadi, S., M. Ebrahimi and E. Shirmohammadi. 2017. Influence of plant-growth-promoting bacteria on germination, growth and nutrients' uptake of Onobrychis sativa L. under drought stress. Journal of Plant Interactions 12: 200-208.
Fonseca, P., R. Moreno and F. Rojo. 2011. Growth of Pseudomonas putida at low temperature: global transcriptomic and proteomic analyses. Environmental Microbiology Reports 3: 329-339.
Foolad, M.R. 2004. Recent advances in genetics of salt tolerance in tomato. Plant Cell Tissue and Organ Culture 76: 101-119.
Ghorbanpour, M., M. Hatami and K. Khavazi. 2013. Role of plant growth promoting rhizobacteria on antioxidant enzyme activities and tropane alkaloid production of Hyoscyamus niger under water deficit stress. Turkish Journal of Biology 37: 350-360.
Gravel, V., H. Antoun and RJ. Tweddell. 2007. Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride: Possible role of indole acetic acid (IAA). Soil Biology and Biochemistry 39: 1968-1977.
Heath, R.L. and L. Packer. 1968. Photoperoxidation in isolated chloroplasts. I. kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125: 189-198.
Heidari, M. and A. Golpayegani. 2012. Effects of water stress and inoculation with plant growth promoting rhizobacteria (PGPR) on antioxidant status and photosynthetic pigments in basil (Ocimum basilicum L.). Journal of The Saudi Sociecty of Agricultural Sciences 11: 57-61.
Kim, T.H., M. Böhmer, H. Hu, N. Nishimura and J.I. Schroeder. 2010. Guard cell signal transduction network: advances in understanding abscisic acid, CO2, and Ca2+ signaling. Annual Review of Plant Biology 61: 561-591.
Kohler, J., J.A. Hernandez, F. Caravaca and A. Roldàn. 2008. Plant-growth-promoting rhizo-bacteria and abuscular mycorrhizal fungi modify alleviation biochemical mechanisms in water-stressed plants. Functional Plant Biology 35: 141-151.
Kuklinsky-Sobral, J., W.L. Araújo, R. Mendes, A.A. Pizzirani-Kleiner and J.L. Azevedo. 2005. Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide. Plant and Soil 273: 91-99.
Li, F., R. Vallabhaneni, J. Yu, T. Rocheford and E.T. Wurtzel. 2008. The maize phytoene synthase gene family: overlapping roles for carotenogenesis in endosperm, photo-morphogenesis, and thermal stress tolerance. Plant Physiology 147: 1334-1346.
Lu, S., W. Su, H Li and Z. Guo. 2009. Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2-and NO-induced antioxidant enzyme activities. Plant Physiology and Biochemistry 47: 132-138.
Ludlow, M.M. 1980. Adaptive significance of stomatal responses to water stress. In: N.C.A. Turner and P.J. Kramer (eds.). Adaptation of Plants to Water and High Temperature Stress. Wiley, New York. pp.123-138.
Mandyal, P., R. Kaushal, K. Sharma and M. Kaushal. 2012. Evaluation of native PGPR isolates in bell pepper for enhanced growth, yield and fruit quality. International Journal Farm Sciences 2: 28-35.
Martínez, G.A., P.M. Civello, A.R. Chaves and M.C. Añón. 2001. Characterization of peroxidase-mediated chlorophyll bleaching in strawberry fruit. Phytochemistry 58: 379-387.
Marulanda, A., J.M. Barea and R. Azcon. 2009. Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. Journal of Plant Growth Regulation 28: 115-124.
Mayak, S., T. Tirosh and B.R. Glick. 2004. Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Science 166: 525-530.
Mezzetti, B., L. Landi, T. Pandolfini and A. Spena. 2004. The defH9-iaaM auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberry. BMC Biotechnology 4: 4.
Mika, A. and S. Lüthje. 2003. Properties of guiacol peroxidase activities isolated from corn root plasma membranes. Plant Physiology 132: 1489-1498.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405-410.
Mohammadi, H., R. Dashi, M. Farzaneh, L. Parviz and H. Hashempour. 2017. Effects of beneficial root pseudomonas on morphological, physiological, and phytochemical characteristics of Satureja hortensis (Lamiaceae) under water stress. Brazilian Journal of Botany 40: 41-48.
Molina-Romero, D., A. Baez, V. Quintero-Hernández, M. Castañeda-Lucio, L.E. Fuentes-Ramírez, M.D.R. Bustillos-Cristales et al. 2017. Compatible bacterial mixture, tolerant to desiccation, improves maize plant growth. Plos One 12: E0187913.
Naamala, J. and D.L. Smith. 2020. Relevance of plant growth promoting microorganisms and their derived compounds, in the face of climate change. Agronomy 10: 1179.
Nakano, Y. and K. Asada. 1987. Purification of ascorbate peroxidase in spinach chloroplasts - its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant and Cell Physiol. 28: 131-140.
Nuruddin M.M., C.A. Madramootoo and GT. Dodds. 2003. Effects of water stress at different growth stages on greenhouse tomato yield and quality. HortScience 38: 1389-1393.
Planchamp, C., G. Glauser and B. Mauch-Mani. 2014. Root inoculation with Pseudomonas putida KT2440 induces transcriptional and metabolic changes and systemic resistance in maize plants. Frontiers in Plant Science 5: 719.
Poblete-Castro, I., D. Binger, R. Oehlert and M. Rohde. 2014. Comparison of mcl-Poly(3-hydroxyalkanoates) synthesis by different Pseudomonas putida strains from crude glycerol: citrate accumulates at high titer under PHA-producing conditions. BMC Bio-technology 14: 962.
Pospisilova, J. 2003. Participation of phytohormones in the stomatal regulation of gas exchange during water stress. Biologia Plantarum 46: 491-506.
Rahman, A., K. Nahar, J. Al Mahmud, M. Hasanuzzaman, M.S. Hossain and M. Fujita. 2017. Salt stress tolerance in rice: emerging role of exogenous phytoprotectants. In: J. Li (ed.). Advances in International Rice Research. InTech, Rijeka. pp. 139-174.
Ramírez, D.A., W. Yactayo, R. Gutiérrez, V. Mares, F. De Mendiburu, A. Posadas and R. Quiroz. 2014. Chlorophyll concentration in leaves is an indicator of potato tuber yield in water-shortage conditions. Scientia Horti-culturae 168: 202-209.
Ruiz-Sola, M., V. Arbona, A. Gomez-Cadenas, M. Rodriguez-Concepcion and A. Rodriguez-Villalon. 2014. A root specific induction of carotenoid biosynthesis contributes to ABA production upon salt stress in Arabidopsis. Plos One 9: e90765.
Ryu, C.M., M.A. Farag, C.H. Hu, M.S. Reddy, H.X. Wei, P.W. Pare and J.W. Kloepper. 2003. Bacterial volatiles promote growth in Arabidopsis. Proc. National Academy of Sciences of USA 100: 4927-4932.
Taheri, P., I. Abdoljabbar, M. Goldani and S. Tarighi. 2014. Oxidative burst and enzymatic antioxidant systems in rice plants during interaction with Alternaria alternata. European Journal of Plant Pathology 140: 829-839.
Tamburino, R., M. Vitale, A. Ruggiero, M. Sassi, L. Sannino, S. Arena et al. 2017. Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biology 17: 40.
Timmusk, S., L. Behers, J. Muthoni, A. Muraya and A.C. Aronsson. 2017. Perspectives and challenges of microbial application for crop improvement. Frontiers in Plant Science 8: 49.
Velikova, V., I. Yordanov and A. Edreva. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science 151: 59-66.
Vilchez, J.I., C. Garcia-Fontana, D. Román-Naranjo, J. González-López and M. Manzanera. 2016. Plant drought tolerance enhancement by trehalose production of desiccation-tolerant microorganisms. Frontiers in Microbiology 7: 1577.
Vivas, A., A. Marulanda, J. Ruiz-Lozano, M.J.M. Barea and R. Azcon. 2003. Influence of a Bacillus sp. on physiological activities of two arbuscular mycorrhizal fungi and on plant responses to PEG induced drought stress. Mycorrhiza 13: 249-256.
Vurukonda, S.S., S. Vardharajula, M. Shrivastava and A . SkZ. 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research 184: 13-24.
Wang, C.J., Y.H. Guo, C. Wang, H.X. Liu, D.D. Niu, Y.P. Wang and J.H. Guo. 2012. Enhancement of tomato (Lycopersicon esculentum) tolerance to drought stress by plant-growth-promoting rhizobacterium (PGPR) Bacillus cereus AR156. Journal of Agricultural Biotechnology 20: 1097-1105.
Wang, X., M. Vignjevic, D. Jiang, S. Jacobsen and B. Wollenweber. 2014. Improved tolerance to drought stress after anthesis due to priming before anthesis in wheat (Triticum aestivum L.) var. Vinjett. Journal of Experimental Botany 65: 6441-6456.
Wu, X., S. Monchy, S. Taghavi, W. Zhu, J. Ramos and D. Van Der Lelie. 2011. Comparative genomics and functional analysis of niche-specific adaptation in Pseudomonas putida. FEMS Microbiology Reviews 35: 299-323.
Yang, J., J.W. Kloepper and C.M. Ryu. 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends in Plant Science 14: 1-4.
Yazici, I., I. Turkan, A.H. Sekmen and T. Demiral. 2007. Salinity tolerance of purslane (Portulaca oleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Environmental and Experimental Botany 61: 49-57.
Yilmaz, S., R. Temizgül, C. Yürürdurmaz and M. Kaplan. 2020. Oxidant and antioxidant enzyme response of redbine sweet sorghum under NaCl salinity stress. Bioagro 32(1): 31-38.
Yuan, X. K., Z. Q. Yang, Y. X. Li, Q. Liu and W. Han. 2016. Effects of different levels of water stress on leaf photosynthetic characteristics and antioxidant enzyme activities of greenhouse tomato. Photosynthetica 54: 28-39.
Zhang, L., G. Ma, M. Kato, K. Yamawaki, T. Takagi, Y. Kiriiwa et al. 2012. Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro. Journal of Experimental Botany 63: 871-886.
Zhang, X., L. Zhang, F.C. Dong, J.F. Gao, D.W. Galbraith and C.P. Song. 2001. Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiology 126: 1438-1448.
Publicado
Cómo citar
Número
Sección
Está obra está bajo licencia Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Derechos del/de autor/es a partir del año de publicación
Esta obra está bajo la licencia:
Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
Las opiniones expresadas por los autores no necesariamente reflejan la postura del editor de la publicación ni de la UCLA. Se autoriza la reproducción total o parcial de los textos aquí publicados, siempre y cuando se cite la fuente completa y la dirección electrónica de esta revista. Los autores(as) tienen el derecho de utilizar sus artículos para cualquier propósito siempre y cuando se realice sin fines de lucro. Los autores(as) pueden publicar en internet o cualquier otro medio la versión final aprobada de su trabajo, luego que esta ha sido publicada en esta revista.
Bioagro se reserva el derecho de realizar modificaciones textuales y ajustes técnicos a las figuras de los manuscritos, de acuerdo con el estilo y especificaciones de la revista.
