THE ROLE OF SILICON TO INCREASE ARSENIC TOLERANCE IN RICE (Oryza sativa L.) SEEDLINGS BY REINFORCING ANTI-OXIDATIVE DEFENSE
Keywords:
Antioxidants, enzymes, plant resistance, toxic elementAbstract
Arsenic is a toxic metalloid which can cause severe problems to plants. On the other hand, silicon is a beneficial element, which supports plants to build resistance under stressed conditions. The objective of the present study was to assess the effect of silicon and arsenic on the various enzymatic, and non-enzymatic antioxidants, in shoots and roots of two rice seedlings (Du-WT and DU-OE), for one and two weeks. Seedlings were exposed to four different culture media: a) Control; b) 0.70 mM Si+no As; c) 30 μM As+no Si; d) 30 μM As+0.70 mM Si. Culture media and rice genotypes were arranged in a 8-treatment factorial with three replications. Results showed that response to silicon, arsenic and or combination of them in unstressed rice plants followed similar patterns, and varied depending upon the antioxidant. The addition of As always decreased the values, but together with silicon there was a partial recovery of them. The pattern of plant response was similar regardless the plant tissue or time of exposure to As. Transgenic Dullar rice, under As stress conditions, activated the highest level of antioxidants, especially when seedlings were treated with silicon.
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2. Ando, K., M. Honma, S. Chiba, S. Tahara and J. Mizutani. 1988. Glutathione transferase from Mucor javanicus. J. Agricultural Biological Chemistry 52: 135-139.
3. Azevedo, M.M., A. Carvalho, C. Pascoal, F. Rodrigues and F. Cássio. 2007. Responses of antioxidant defenses to Cu and Zn stress in two aquatic fungi. J. Science of the Total Environment 377: 233-243.
4. Begum, M.C., M.S. Islam, M. Islam, R. Amin, M.S. Parvez and A.H. Kabir. 2016. Biochemical and molecular responses underlying differential arsenic tolerance in rice (Oryza sativa L.). J. Plant Physiology Biochemistry 104: 266-277.
5. Cobbett, C. and P. Goldsbrough. 2002. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annual Review of Plant Biology 53: 159-182.
6. Cummins, I., D.P. Dixon, S. Freitag-Pohl, M. Skipsey and R. Edwards. 2011. Multiple roles for plant glutathione transferases in xenobiotic detoxification. Drug Metabolism Reviews 43: 266-280.
7. Ding, N., A. Wang, X. Zhang, Y. Wu, R. Wang, H. Cui et al. 2017. Identification and analysis of glutathione S-transferase gene family in sweet potato reveal divergent GST-mediated networks in aboveground and underground tissues in response to abiotic stresses. J BMC Plant Biology 17: 225.
8. Esposito, J.B.N., B.P. Esposito, R.A. Azevedo, L.S. Cruz, L.C. da Silva and S.R. de Souza. 2015. Protective effect of Mn (III)–desferrioxamine B upon oxidative stress caused by ozone and acid rain in the Brazilian soybean cultivar Glycine max “Sambaiba”. J. Environmental Science 22: 5315-5324.
9. Geng, A., X. Wang, L. Wu, F. Wang, Z. Wu, H. Yang et al..2018. Silicon improves growth and alleviates oxidative stress in rice seedlings (Oryza sativa L.) by strengthening antioxidant defense and enhancing protein metabolism under arsanilic acid exposure. J. Ecotoxicology Environmental Safety 158: 266-273.
10. Gill, S.S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. J. Plant Physiology Biochemistry 48: 909-930.
11. He, J., F. Chen, S. Chen, G. Lv, Y. Deng, W. Fang et al. 2011. Chrysanthemum leaf epidermal surface morphology and antioxidant and defense enzyme activity in response to aphid infestation. J. Plant Physiology 168: 687-693.
12. Heath, R. and L. Packer. 1968. Photoper-oxidation in isolated chloroplast. I. Kinetics stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125(1): 189-98.
13. Ju, S., N. Yin, L. Wang, C. Zhang and Y. Wang. 2017. Effects of silicon on Oryza sativa L. seedling roots under simulated acid rain stress. PloS One 12(3): e0173378.
14. Kang, J., W. Zhao and X. Zhu. 2016. Silicon improves photosynthesis and strengthens enzyme activities in the C3 succulent xerophyte Zygophyllum xanthoxylum under drought stress. J. Plant Physiology 199: 76-86.
15. Khan, E. and M. Gupta. 2018. Arsenic-silicon priming of rice (Oryza sativa L.) seeds influence mineral nutrient uptake and biochemical responses through modulation of Lsi-1, Lsi-2, Lsi-6 and nutrient transporter genes. J. Scientific Reports 8: 1-16.
16. Li-Ping, B., S. Fang-Gong, G. Ti-Da, S. Zhao-Hui, L. Yin-Yan and Z. Guang-Sheng. 2006. Effect of soil drought stress on leaf water status, membrane permeability and enzymatic antioxidant system of maize. Pedosphere 16: 326-332.
17. Liu, T., S. Zhong, X. Liao, J. Chen, T. He, S. Lai and Y. Jia. 2015. A meta-analysis of oxidative stress markers in depression. PloS One 10(10): e0138904.
18. Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. J. Plant Cell Physiology 22: 867-880.
19. Pan, W., C. Wu, S. Xue and W. Hartley. 2014. Arsenic dynamics in the rhizosphere and its sequestration on rice roots as affected by root oxidation. Environ. Sci. 26: 892-899.
20. Rahman, M.F., A. Ghosal, M.F. Alam and A.H. Kabir. 2017. Remediation of cadmium toxicity in field peas (Pisum sativum L.) through exogenous silicon. J Ecotoxicology Environmental Safety 135: 165-172.
21. Rao, G.B., P.Y. PI and E.K. Syriac. 2017. Silicon nutrition in rice: A review. J. Pharmacognosy and Phytochemistry 6(6): 390-392.
22. Raza, M.M., S. Ullah, Z. Ahmad, S. Saqib, S. Ahmad, H.M. Bilal and F. Wali. 2016. Silicon mediated arsenic reduction in rice by limiting its uptake. Agricultural Sciences in China 7: 1.
23. Rodríguez-Lado, L., G. Sun, M. Berg, Q. Zhang, H. Xue, Q. Zheng and C.A. Jonson. 2013. Groundwater arsenic contamination throughout China. Science 341(6148): 866-868.
24. Sajedi, N.A., M.R. Ardakani, H. Madani, A. Naderi and M. Miransari. 2011. The effects of selenium and other micronutrients on the antioxidant activities and yield of corn (Zea mays L.) under drought stress. J. Physiology Molecular Biology of Plants 17: 215-222.
25. Sedlak, J. and R.H. Lindsay. 1968. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. J. Analytical biochemistry 25: 192-205.
26. Shri, M., S. Kumar, D. Chakrabarty, P.K. Trivedi, S. Mallick, P. Misra et al. 2009. Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. J. Ecotoxicology Environmental Safety 72: 1102-1110.
27. Wang, S., F. Wang and S. Gao. 2015. Foliar application with nano-silicon alleviates Cd toxicity in rice seedlings. J. Environmental Science Pollution Research 22: 2837-2845.
28. Xiang, C., B.L. Werner, M.C. E'Lise and D.J. Oliver. 2001. The biological functions of glutathione revisited in Arabidopsis transgenic plants with altered glutathione levels. J. Plant Physiology 126: 564-574.
29. Yoshida, S., D.A. Forno, J. Cock and K. Gomez. 1976. Laboratory Manual for Physiological Studies of Rice: Int. Rice Res. Institute. Los Baños, Philippines. 83 p.
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