Efecto de impregnar la semilla con EDTA en la germinación y atributos bioquímicos en tres líneas de maíz bajo estrés de NaCl

Autores/as

DOI:

https://doi.org/10.51372/bioagro343.3

Palabras clave:

Ácido salicílico, EDTA, salinidad, Zea mays

Resumen

Se ha demostrado que los efectos adversos de la salinidad pueden variar entre distintas variedades de una misma especie vegetal. Este estudio se realizó para determinar la tolerabilidad de tres líneas de maíz recién liberadas a condiciones salinas cuando se impregnaron con ácido etilendiaminotetraacético (EDTA) y ácido salicílico (SA), cada uno a una concentración de 1,0 M; como control se usaron semillas humedecidas con agua destilada. Se probaron concentraciones salinas de 0, 50, 100, 150 y 300 mM de NaCl. El experimento tuvo un arreglo factorial de tratamientos de 3x3x5 con tres repeticiones. Los resultados revelaron una interacción significativa entre las líneas de maíz y la salinidad donde el porcentaje de germinación de la línea SWAN-LSR-Y fue mucho más afectado que las otras líneas al nivel de salinidad de 50 mM, y su velocidad de germinación fue más afectada que las otras al pasar de 150 a 300 mM de NaCl. Plántulas (radícula y plúmula) y el índice de vigor de la semilla fueron influenciados por una interacción significativa entre el agente impregnante y la salinidad, donde EDTA incrementó el crecimiento de las plántulas más que los otros impregnantes cuando la salinidad no sobrepasó l50 mM. La línea de maíz OMR-LSR-SY no mostró tolerabilidad a esta concentración. De manera similar, las líneas de maíz tratadas con EDTA y SA mostraron una menor acumulación de especies reactivas de oxígeno, como el radical anión superóxido (O2-) y el peróxido de hidrógeno (H2O2), así como una disminución en los contenidos de malondialdehído (MDA), principalmente en las líneas SWAN-LSR-Y y BR9928-OMR-SR-Y. Las actividades de la catalasa (CAT) y la superóxido dismutasa (SOD) se incrementaron en SWAN-LSR-Y y BR9928-OMR-SR-Y luego de la aplicación del EDTA.

Descargas

La descarga de datos todavía no está disponible.

Citas

Abdulbaki, A.S., H. Alsamadany and Y. Alzahrani. 2019. β-Aminobutyric acid (BABA) priming and abiotic stresses. International Journal of Bioscience 14: 450-459.

Afolabi, O.B., O.I. Oloyede, I.I. Olayide, T.O. Obafemi, J.O. Awe, B.A. Afolabi and S.A. Onikani. 2015. Antioxidant enhancing ability of different solvents extractable components of Talinum triangulare in some selected tissue homogenates of albino rats in vitro. Journal of Applied Pharmaceutical Science 5: 56-61.

Ahanger, M.A. and R.M. Agarwal. 2017. Salinity stress induced alterations in antioxidant metabolism and nitrogen assimilation in wheat (Triticum aestivum L) as influenced by potassium supplementation. Plant Physiology and Biochemistry 115: 449-460.

Ahmad, F., A. Kamal, A. Singh, F. Ashfaque, S. Alamri, M.H. Siddiqui and M.I.R. Khan. 2021. Seed priming with gibberellic acid induces high salinity tolerance in Pisum sativum through antioxidants, secondary metabolites and up‐regulation of antiporter genes. Plant Biology 23: 113-121.

Ajiboye, T.O., A.M. Naibi, I.O. Abdulazeez, I.O. Alege, A.O. Mohammed, S.A. Bello and H.F. Muritala. 2016. Involvement of oxidative stress in bactericidal activity of 2-(2-nitrovinyl) furan against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Microbial Pathogenesis 91: 107-114.

Akter, L., O.A. Fakir, M.K. Alam, M. U. Islam, P. Chakraborti, M.J. Alam and M.A. Kader. 2018. Amelioration of salinity stress in maize seed germination and seedling growth attributes through seed priming. Open Journal of Soil Science 8(05): 137.

Aloui, H., M. Souguir, S. Latique and C. Hannachi. 2014. Germination and growth in control and primed seeds of pepper as affected by salt stress. Cercetari Agronomice în Moldova 47: 83-95.

Chimwemwe, B., D. Ketthaisong, P. Pongdontri and D. Jothityangkoon. 2021. Seed priming promotes germination and seedling development of hybrid and open pollinatedcultivars of super sweet corn. Kaen Kaset Journal 46(4): 787-798.

Chiu, K.Y., C.L. Chen and J.M. Sung. 2002. Effect of priming temperature on storability of primed sh‐2 sweet corn seed. Crop Science 42: 1996-2003.

El Sabagh, A., A. Hossain, C. Barutçular, M.A. Iqbal, M.S. Islam, S. Fahad and M. Erman, 2020. Consequences of salinity stress on the quality of crops and its mitigation strategies for sustainable crop production: an outlook of arid and semi-arid regions. In: S. Fahad et al. (eds). Environment, climate, plant and vegetation growth. Springer, Cham. pp. 503-533.

Esper Neto, M., D.W. Britt, K.A. Jackson, C.F. Coneglian, T.T. Inoue and M.A. Batista. 2021. Early growth of corn seedlings after seed priming with magnetite nanoparticles synthetised in easy way. Acta Agriculturae Scandinavica, Section B. Soil & Plant Science, 71(2): 91-97.

Farooq, M., M. Hussain, A. Wakeel and K.H. Siddique. 2015. Salt stress in maize: effects, resistance mechanisms and management. A review. Agronomy for Sustainable Development 35: 461-481.

Farzana, S., M. Rasel, M. Arif, M. Galib, K. Sarker and M. Hossain. 2020. Exogenous salicylic acid and thiourea ameliorate salt stress in wheat by enhancing photosynthetic attributes and antioxidant defense. Journal of Bangladesh Agricultural University 18(2): 272-282.

Galviz-Fajardo, Y.C., G.S. Bortolin, S. Deuner, L.D. Amarante, F. Reolon and D.M. Moraes. 2020. Seed priming with salicylic acid potentiates water restriction-induced effects in tomato seed germination and early seedling growth. Journal of Seed Science 42: 1-12.

Ghafoor, M. F., Q. Ali and A. Malik. 2020. Effects of salicylic acid priming for salt stress tolerance in wheat. Biological and Clinical Sciences Research Journal 2020(1): 1-12.

Ghani, M.A., M.M. Abbas, B. Ali, K. Ziaf, M. Azam, R. Anjum et al. 2021. Role of salicylic acid in heat stress tolerance in tri-genomic Brassica napus L. Bioagro 33(1): 13-20.

Habiba, U., S. Ali, M. Farid, M. B. Shakoor, M. Rizwan, M. Ibrahim and B. Ali. 2015. EDTA enhanced plant growth, antioxidant defense system, and phytoextraction of copper by Brassica napus L. Environmental Science and Pollution Research 22(2): 1534-1544.

Hadwan, M.H. and H.N. Abed. 2016. Data supporting the spectrophotometric method for the estimation of catalase activity. Data in Brief 6: 194-199.

Hussain S., F. Khan, H.A. Hussain and L. Nie. 2016. Physiological and biochemical mechanisms of seed priming-induced chilling tolerance in rice cultivars. Frontiers in Plant Science 7: 116.

Iqbal, S., Q. Ali and A. Malik. 2021. Effects of seed priming with salicylic acid on zea mays seedlings grown under salt stress conditions. Biological and Clinical Sciences Research Journal 2021(1):1-10.

Kang, G., G. Li, B. Zheng, Q. Han, C. Wang, Y. Zhu and T. Guo. 2012. Proteomic analysis on salicylic acid-induced salt tolerance in common wheat seedlings (Triticum aestivum L.). Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1824(12): 1324-1333.

Liting, W., W. Lina, Y. Yang, W. Pengfei, G. Tiancai and K. Guozhang. 2015. Abscisic acid enhances tolerance of wheat seedlings to drought and regulates transcript levels of genes encoding ascorbate-glutathione biosynthesis. Frontiers in Plant Science 6: 1-11.

Maas, E.V. and G.J. Hoffman. 1977. Crop salt tolerance—current assessment. Journal of the Irrigation and Drainage Division 103: 115-134.

Maguire, J.D. 1962. Speed of germination. Aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2: 176-177.

Maisuria, K.M and S.T. Patel. 2009. Seed germinability, root and shoot length and vigour index of soybean as influenced by rhizosphere fungi. Karnataka Journal of Agricultural Sciences 22(5): 1120-1122.

Mgbeze, G.C., J.O. Omodamwen and J.M. Okuo. 2011. The effects of salinity and ethylenedamine tetra acetic acid (EDTA) on germination of two species of pepper (Capsicum annum L. and Capsicum frutescens L.). Nigeria Journal of Botany 24(1): 91-98.

Nabavi Kalat, S.M., M. Ghasemi and A. Kelidari. 2022. Effect of deficit irrigation stress and plant density on antioxidant‎ enzymes activity, compatible osmolytes, relative water content and‎ yield of baby corn (Pashan cultivar)‎. Iranian Journal of Irrigation & Drainage 15(6): 1370-1381.

Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 22: 867-880.

Olayinka, B.U., E.T. Ayanduro, A.A. Abdulrahaman and E.O. Etejere. 2016. Effects of salinity and ethylenediamine tetra acetic acid (EDTA) on the germination of tomato (Solanum lycopersicum L.) seeds. Science World Journal 11(4): 1-3.

Pire, R. and G. Vargas-Simón. 2019. Recurrent inconsistencies in publications that involve Maguire’s germination rate formula. Forest Systems 28(1): eSC02, 5 p.

Raja, V., U.M. Wani, Z.A. Wani, N. Jan, C. Kottakota, M.K. Reddy and R. John. 2021. Pyramiding ascorbate–glutathione pathway in Lycopersicum esculentum confers tolerance to drought and salinity stress. Plant Cell Reports 2021: 1-19.

Reilly, C.A. and S.D. Aust. 2001. Measurement of lipid peroxidation. Current Protocol in Toxicology 22: 412-413.

Sabagh, A.E., F. Çiğ, S. Seydoşoğlu, M.L. Battaglia, T. Javed, M.A. Iqbal and M. Awad, 2021. Salinity stress in maize: Effects of stress and recent developments of tolerance for improvement. Cereal Grains 1: 213.

Sahab, S., I. Suhani, V. Srivastava, P.S. Chauhan, R.P. Singh and V. Prasad. 2021. Potential risk assessment of soil salinity to agroecosystem sustainability: Current status and management strategies. Science of the Total Environment 764: 144164.

Shah, T., S. Latif, F. Saeed, I. Ali, S. Ullah, A.A. Alsahli and P. Ahmad. 2021. Seed priming with titanium dioxide nanoparticles enhances seed vigor, leaf water status, and antioxidant enzyme activities in maize (Zea mays L.) under salinity stress. Journal of King Saud University-Science 33(1):101207.

Sharma, R.K. 2012. Effect of salicylic acid and gibberellic acid on seed germination and growth of pea. International Journal of Plant Sciences (Muzaffarnagar) 7(2): 322-324.

Tian, Y., B. Guan, D. Zhou, J. Yu, G. Li and Y. Lou. 2014. Responses of seed germination, seedling growth, and seed yield traits to seed pretreatment in maize (Zea mays L.). The Scientific World Journal 2014: 1-9.

Valenzuela, F. J., D. Reineke, D. Leventini, C. C. L. Chen, E. G. Barrett-Lennard, T. D. Colmer and N. Bazihizina. 2022. Plant responses to heterogeneous salinity: agronomic relevance and research priorities. Annals of Botany 20: 1-19.

Velikova V., I. Yordanov, and A. Edreva. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Science 151: 59-66.

Younesi O. and A. Moradi. 2014. Effect of priming of seeds of Medicago sativa" Bami" with gibberellic acid on germination, seedlings growth and antioxidant enzymes activity under salinity stress. Journal of Horticultural Research 22(2): 167-174.

Zhu, T., L. Li, Q. Duan, X. Liu and M. Chen. 2021. Progress in our understanding of plant responses to the stress of heavy metal cadmium. Plant Signaling & Behavior 16(1): 1836884.

Publicado

2022-08-31

Cómo citar

Umar Olayinka, B., Abdulkareem, K., Abdulbaki, A., Alsamadany, H., Alzahrani, Y., Isiaka, K., Ayinla, A., Kolawole, O., Idowu, A., Odudu, F., Ibuowo, M., Mustapha, O., & Sulyman, A. (2022). Efecto de impregnar la semilla con EDTA en la germinación y atributos bioquímicos en tres líneas de maíz bajo estrés de NaCl. Bioagro, 34(3), 233-244. https://doi.org/10.51372/bioagro343.3